View source for 6a8l


==Crystal structure of nicotinamidase/ pyrazinamidase PncA from Bacillus subtilis==
<StructureSection load='6a8l' size='340' side='right'caption='[[6a8l]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6a8l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A8L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A8L FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a8l OCA], [https://pdbe.org/6a8l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a8l RCSB], [https://www.ebi.ac.uk/pdbsum/6a8l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a8l ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A223CGG0_9BACI A0A223CGG0_9BACI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The nicotinamidase/pyrazinamidase PncA is a member of a large family of hydrolase enzymes that catalyze the deamination of nicotinamide to nicotinic acid. PncA also functions as a pyrazinamidase in a wide variety of eubacteria and is an essential coenzyme in many cellular redox reactions in living systems. We report the crystal structure of substrate-free PncA from Bacillus subtilis (BsPncA) at 2.0A resolution to improve our understanding of the PncA family. The structure of BsPncA consists of an alpha/beta domain and a subdomain. The subdomain of BsPncA has a different conformation than that of PncA enzymes from other organisms. The B-factor analysis revealed a rigid structure of the alpha/beta domain, while the subdomain is highly flexible. Both dimers and tetramers were observed in BsPncA protein crystals, but only dimers were observed in solution. Our results provide useful information that will further enhance our understanding of the molecular functions of PncA family members.

Crystal structure of the nicotinamidase/pyrazinamidase PncA from Bacillus subtilis.,Shang F, Chen J, Wang L, Jin L, Zou L, Bu T, Dong Y, Ha NC, Nam KH, Quan C, Xu Y Biochem Biophys Res Commun. 2018 Aug 11. pii: S0006-291X(18)31749-2. doi:, 10.1016/j.bbrc.2018.08.067. PMID:30107912<ref>PMID:30107912</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6a8l" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Chen J]]
[[Category: Shang F]]
[[Category: Wang L]]
[[Category: Xu Y]]