View source for 5tme


==Crystal structure of Os79 from O. sativa in complex with UDP.==
<StructureSection load='5tme' size='340' side='right'caption='[[5tme]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5tme]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TME FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tme OCA], [https://pdbe.org/5tme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tme RCSB], [https://www.ebi.ac.uk/pdbsum/5tme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tme ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/UGT79_ORYSJ UGT79_ORYSJ] Involved in the detoxification of the Fusarium mycotoxin deoxynivalenol by the transfer of glucose from UDP-D-glucose to the hydroxyl group at C-3, forming deoxynivalenol-3-O-beta-D-glucoside.<ref>PMID:26197338</ref> <ref>PMID:27715009</ref> 
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== Publication Abstract from PubMed ==
Fusarium head blight is a plant disease with significant agricultural and health impacts which affects cereal crops such as wheat, barley, and maize, and is characterized by reduced grain yield and the accumulation of trichothecene mycotoxins such as deoxynivalenol (DON). Studies have identified trichothecene production as a virulence factor in Fusarium graminearum, and have linked DON resistance to the ability to form DON-3-O-glucoside in wheat. Here, the structures of a deoxynivalenol:UDP-glucosyltransferase (Os79) from Oryza sativa are reported in complex with UDP in an open conformation, in complex with UDP in a closed conformation, and in complex with UDP-2-fluoro-2-deoxy-D-glucose and trichothecene at 1.8, 2.3, and 2.2 A resolution respectively. The active site of Os79 lies in a groove between the N-terminal acceptor and the C-terminal donor-binding domains. Structural alignments reveal that Os79 likely utilizes a catalytic mechanism similar to other plant UGTs, with His 27 activating the trichothecene O3 hydroxyl for nucleophilic attack at C1 of the UDP-glucose donor. Kinetic analysis of mutant Os79 revealed that Thr 291 plays a critical role in catalysis as either a catalytic acid or to position the UDP moiety during the nucleophilic attack. Steady-state kinetic analysis demonstrated that Os79 conjugates multiple trichothecene substrates such as DON, nivalenol, isotrichodermol, and HT-2 toxin, but not T-2 toxin. These data establish a foundation for understanding substrate specificity and activity in this enzyme, and can be used to guide future efforts to increase DON resistance in cereal crops.

Crystal structure of Os79 (Os04g0206600) from Oryza sativa: A UDP-glucosyltransferase involved in the detoxification of deoxynivalenol.,Wetterhorn KM, Newmister SA, Caniza RK, Busman M, McCormick SP, Berthiller F, Adam G, Rayment I Biochemistry. 2016 Oct 7. PMID:27715009<ref>PMID:27715009</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5tme" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Oryza sativa Japonica Group]]
[[Category: Adam G]]
[[Category: Berthiller F]]
[[Category: Busman M]]
[[Category: Caniza RK]]
[[Category: McCormick SP]]
[[Category: Newmister SA]]
[[Category: Rayment I]]
[[Category: Wetterhorn KM]]