View source for 5t38


==Crystal Structure of the N-terminal domain of EvdMO1 with SAH bound==
<StructureSection load='5t38' size='340' side='right'caption='[[5t38]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5t38]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_carbonacea Micromonospora carbonacea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T38 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1502&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t38 OCA], [https://pdbe.org/5t38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t38 RCSB], [https://www.ebi.ac.uk/pdbsum/5t38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t38 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1C5AE13_9ACTN A0A1C5AE13_9ACTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Members of the orthosomycin family of natural products are decorated polysaccharides with potent antibiotic activity and complex biosynthetic pathways. The defining feature of the orthosomycins is an orthoester linkage between carbohydrate moieties that is necessary for antibiotic activity and is likely formed by a family of conserved oxygenases. Everninomicins are octasaccharide orthosomycins produced by Micromonospora carbonacea that have two orthoester linkages and a methylenedioxy bridge, three features whose formation logically requires oxidative chemistry. Correspondingly, the evd gene cluster encoding everninomicin D encodes two monofunctional nonheme iron, alpha-ketoglutarate-dependent oxygenases and one bifunctional enzyme with an N-terminal methyltransferase domain and a C-terminal oxygenase domain. To investigate whether the activities of these domains are linked in the bifunctional enzyme EvdMO1, we determined the structure of the N-terminal methyltransferase domain to 1.1 A and that of the full-length protein to 3.35 A resolution. Both domains of EvdMO1 adopt the canonical folds of their respective superfamilies and are connected by a short linker. Each domain's active site is oriented such that it faces away from the other domain, and there is no evidence of a channel connecting the two. Our results support EvdMO1 working as a bifunctional enzyme with independent catalytic activities.

The Structure of the Bifunctional Everninomicin Biosynthetic Enzyme EvdMO1 Suggests Independent Activity of the Fused Methyltransferase-Oxidase Domains.,Starbird CA, Perry NA, Chen Q, Berndt S, Yamakawa I, Loukachevitch LV, Limbrick EM, Bachmann BO, Iverson TM, McCulloch KM Biochemistry. 2018 Dec 18;57(50):6827-6837. doi: 10.1021/acs.biochem.8b00836., Epub 2018 Dec 7. PMID:30525509<ref>PMID:30525509</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5t38" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Micromonospora carbonacea]]
[[Category: Berndt S]]
[[Category: Chen Q]]
[[Category: Iverson TM]]
[[Category: Loukachevitch LV]]
[[Category: McCulloch KM]]
[[Category: Perry NA]]
[[Category: Starbird C]]
[[Category: Yamakawa I]]