View source for 5nb4


==Atomic resolution structure of C-phycoerythrin from marine cyanobacterium Phormidium sp. A09DM at pH 7.5==
<StructureSection load='5nb4' size='340' side='right'caption='[[5nb4]], [[Resolution|resolution]] 1.14&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5nb4]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Phormidium_rubidum_A09DM Phormidium rubidum A09DM]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NB4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.14&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PEB:PHYCOERYTHROBILIN'>PEB</scene>, <scene name='pdbligand=PI:HYDROGENPHOSPHATE+ION'>PI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nb4 OCA], [https://pdbe.org/5nb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nb4 RCSB], [https://www.ebi.ac.uk/pdbsum/5nb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nb4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0E3W010_9CYAN A0A0E3W010_9CYAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
C-Phycoerythrin (PE) from Phormidium sp. A09DM has been crystallized using different conditions and its structure determined to atomic resolution (1.14 A). In order for the pigment present, phycoerythrobilin (PEB), to function as an efficient light-harvesting molecule it must be held rigidly (Kupka and Scheer in Biochim Biophys Acta 1777:94-103, 2008) and, moreover, the different PEB molecules in PE must be arranged, relative to each other, so as to promote efficient energy transfer between them. This improved structure has allowed us to define in great detail the structure of the PEBs and their binding sites. These precise structural details will facilitate theoretical calculations of each PEB's spectroscopic properties. It was possible, however, to suggest a model for which chromophores contribute to the different regions of absorption spectrum and propose a tentative scheme for energy transfer. We show that some subtle differences in one of these PEB binding sites in two of the 12 subunits are caused by crystal contacts between neighboring hexamers in the crystal lattice. This explains some of the differences seen in previous lower resolution structures determined at two different pH values (Kumar et al. in Photosyn Res 129:17-28, 2016).

An improved crystal structure of C-phycoerythrin from the marine cyanobacterium Phormidium sp. A09DM.,Sonani RR, Roszak AW, Ortmann de Percin Northumberland C, Madamwar D, Cogdell RJ Photosynth Res. 2017 Sep 16. doi: 10.1007/s11120-017-0443-2. PMID:28918447<ref>PMID:28918447</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5nb4" style="background-color:#fffaf0;"></div>

==See Also==
*[[Phycocyanin|Phycocyanin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Phormidium rubidum A09DM]]
[[Category: Cogdell RJ]]
[[Category: Madamwar D]]
[[Category: Ortmann de Percin Northumberland C]]
[[Category: Roszak AW]]
[[Category: Sonani RR]]