View source for 5m94


==Crystal structure of Staphylococcus capitis divalent metal ion transporter (DMT) in complex with nanobody==
<StructureSection load='5m94' size='340' side='right'caption='[[5m94]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5m94]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama] and [https://en.wikipedia.org/wiki/Staphylococcus_capitis Staphylococcus capitis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4wgv 4wgv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M94 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m94 OCA], [https://pdbe.org/5m94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m94 RCSB], [https://www.ebi.ac.uk/pdbsum/5m94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m94 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Members of the SLC11 (NRAMP) family transport iron and other transition-metal ions across cellular membranes. These membrane proteins are present in all kingdoms of life with a high degree of sequence conservation. To gain insight into the determinants of ion selectivity, we have determined the crystal structure of Staphylococcus capitis DMT (ScaDMT), a close prokaryotic homolog of the family. ScaDMT shows a familiar architecture that was previously identified in the amino acid permease LeuT. The protein adopts an inward-facing conformation with a substrate-binding site located in the center of the transporter. This site is composed of conserved residues, which coordinate Mn2+, Fe2+ and Cd2+ but not Ca2+. Mutations of interacting residues affect ion binding and transport in both ScaDMT and human DMT1. Our study thus reveals a conserved mechanism for transition-metal ion selectivity within the SLC11 family.

Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.,Ehrnstorfer IA, Geertsma ER, Pardon E, Steyaert J, Dutzler R Nat Struct Mol Biol. 2014 Oct 19. doi: 10.1038/nsmb.2904. PMID:25326704<ref>PMID:25326704</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5m94" style="background-color:#fffaf0;"></div>

==See Also==
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: Staphylococcus capitis]]
[[Category: Dutzler R]]
[[Category: Ehrnstorfer IA]]