View source for 5flx


==Mammalian 40S HCV-IRES complex==
<SX load='5flx' size='340' side='right' viewer='molstar' caption='[[5flx]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5flx]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FLX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5flx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5flx OCA], [https://pdbe.org/5flx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5flx RCSB], [https://www.ebi.ac.uk/pdbsum/5flx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5flx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS3_HUMAN RS3_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Internal ribosomal entry sites (IRESs) are structured cis-acting RNAs that drive an alternative, cap-independent translation initiation pathway. They are used by many viruses to hijack the translational machinery of the host cell. IRESs facilitate translation initiation by recruiting and actively manipulating the eukaryotic ribosome using only a subset of canonical initiation factor and IRES transacting factors. Here we present cryo-EM reconstructions of the ribosome 80S- and 40S-bound Hepatitis C Virus (HCV) IRES. The presence of four subpopulations for the 80S*HCV IRES complex reveals dynamic conformational modes of the complex. At a global resolution of 3.9 A for the most stable complex, a derived atomic model reveals a complex fold of the IRES RNA and molecular details of its interaction with the ribosome. The comparison of obtained structures explains how a modular architecture facilitates mRNA loading and tRNA binding to the P-site. This information provides the structural foundation for understanding the mechanism of HCV IRES RNA-driven translation initiation.

Molecular architecture of the ribosome-bound Hepatitis C Virus internal ribosomal entry site RNA.,Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CM EMBO J. 2015 Dec 14;34(24):3042-58. doi: 10.15252/embj.201592469. Epub 2015 Nov, 24. PMID:26604301<ref>PMID:26604301</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5flx" style="background-color:#fffaf0;"></div>

==See Also==
*[[3D sructureseceptor for activated protein kinase C 1|3D sructureseceptor for activated protein kinase C 1]]
== References ==
<references/>
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</SX>
[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Burger J]]
[[Category: Collier M]]
[[Category: Dabrowski M]]
[[Category: Hilal T]]
[[Category: Hildebrand PW]]
[[Category: Ismer J]]
[[Category: Loerke J]]
[[Category: Mielke T]]
[[Category: Scheerer P]]
[[Category: Schmidt A]]
[[Category: Shaikh TR]]
[[Category: Spahn CMT]]
[[Category: Sprink T]]
[[Category: Yamamoto H]]
[[Category: Yamamoto K]]