View source for 5f5n


==The structure of monooxygenase KstA11 in complex with NAD and its substrate==
<StructureSection load='5f5n' size='340' side='right'caption='[[5f5n]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5f5n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_sp._TP-A0468 Micromonospora sp. TP-A0468]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F5N FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.304&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5VD:METHYL+(1~{R},2~{R},4~{S})-2-METHYL-2,4,5,7,10-PENTAKIS(OXIDANYL)-6,11-BIS(OXIDANYLIDENE)-3,4-DIHYDRO-1~{H}-TETRACENE-1-CARBOXYLATE'>5VD</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5n OCA], [https://pdbe.org/5f5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f5n RCSB], [https://www.ebi.ac.uk/pdbsum/5f5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5n ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A023GUL3_9ACTN A0A023GUL3_9ACTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ranking among the most effective anticancer drugs, anthracyclines represent an important family of aromatic polyketides generated by type II polyketide synthases (PKSs). After formation of polyketide cores, the post-PKS tailoring modifications endow the scaffold with various structural diversities and biological activities. Here we demonstrate an unprecedented four-enzyme-participated hydroxyl regioisomerization process involved in the biosynthesis of kosinostatin. First, KstA15 and KstA16 function together to catalyze a cryptic hydroxylation of the 4-hydroxyl-anthraquinone core, yielding a 1,4-dihydroxyl product, which undergoes a chemically challenging asymmetric reduction-dearomatization subsequently acted by KstA11; then, KstA10 catalyzes a region-specific reduction concomitant with dehydration to afford the 1-hydroxyl anthraquinone. Remarkably, the shunt product identifications of both hydroxylation and reduction-dehydration reactions, the crystal structure of KstA11 with bound substrate and cofactor, and isotope incorporation experiments reveal mechanistic insights into the redox dearomatization and rearomatization steps. These findings provide a distinguished tailoring paradigm for type II PKS engineering.

Hydroxyl regioisomerization of anthracycline catalyzed by a four-enzyme cascade.,Zhang Z, Gong YK, Zhou Q, Hu Y, Ma HM, Chen YS, Igarashi Y, Pan L, Tang GL Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1554-1559. doi:, 10.1073/pnas.1610097114. Epub 2017 Jan 30. PMID:28137838<ref>PMID:28137838</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5f5n" style="background-color:#fffaf0;"></div>

==See Also==
*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Micromonospora sp. TP-A0468]]
[[Category: Gong Y]]
[[Category: Pan L]]