View source for 5exf


==Thermostable aldehyde dehydrogenase from Pyrobaculum sp.1860 complexed with NADP+==
<StructureSection load='5exf' size='340' side='right'caption='[[5exf]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5exf]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrobaculum_ferrireducens Pyrobaculum ferrireducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EXF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5exf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5exf OCA], [https://pdbe.org/5exf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5exf RCSB], [https://www.ebi.ac.uk/pdbsum/5exf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5exf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G7VCG0_9CREN G7VCG0_9CREN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We present the functional and structural characterization of the first archaeal thermostable NADP-dependent aldehyde dehydrogenase AlDHPyr1147. In vitro, AlDHPyr1147 catalyzes the irreversible oxidation of short aliphatic aldehydes at 60-85 degrees capital ES, Cyrillic, and the affinity of AlDHPyr1147 to the NADP+ at 60 degrees capital ES, Cyrillic is comparable to that for mesophilic analogues at 25 degrees capital ES, Cyrillic. We determined the structures of the apo form of AlDHPyr1147 (3.04 A resolution), three binary complexes with the coenzyme (1.90, 2.06, and 2.19 A), and the ternary complex with the coenzyme and isobutyraldehyde as a substrate (2.66 A). The nicotinamide moiety of the coenzyme is disordered in two binary complexes, while it is ordered in the ternary complex, as well as in the binary complex obtained after additional soaking with the substrate. AlDHPyr1147 structures demonstrate the strengthening of the dimeric contact (as compared with the analogues) and the concerted conformational flexibility of catalytic Cys287 and Glu253, as well as Leu254 and the nicotinamide moiety of the coenzyme. A comparison of the active sites of AlDHPyr1147 and dehydrogenases characterized earlier suggests that proton relay systems, which were previously proposed for dehydrogenases of this family, are blocked in AlDHPyr1147, and the proton release in the latter can occur through the substrate channel.

NADP-Dependent Aldehyde Dehydrogenase from Archaeon Pyrobaculum sp.1860: Structural and Functional Features.,Bezsudnova EY, Petrova TE, Artemova NV, Boyko KM, Shabalin IG, Rakitina TV, Polyakov KM, Popov VO Archaea. 2016 Nov 10;2016:9127857. doi: 10.1155/2016/9127857. eCollection 2016. PMID:27956891<ref>PMID:27956891</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5exf" style="background-color:#fffaf0;"></div>

==See Also==
*[[Aldehyde dehydrogenase 3D structures|Aldehyde dehydrogenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pyrobaculum ferrireducens]]
[[Category: Bezsudnova EY]]
[[Category: Boyko KM]]
[[Category: Nikolaeva AY]]
[[Category: Petrova T]]
[[Category: Popov VO]]
[[Category: Rakitina TV]]