View source for 5ahv


==Cryo-EM structure of helical ANTH and ENTH tubules on PI(4,5)P2-containing membranes==
<SX load='5ahv' size='340' side='right' viewer='molstar' caption='[[5ahv]], [[Resolution|resolution]] 13.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ahv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AHV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ahv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ahv OCA], [https://pdbe.org/5ahv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ahv RCSB], [https://www.ebi.ac.uk/pdbsum/5ahv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ahv ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/ENT1_YEAST ENT1_YEAST] Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin. Negatively regulated via phosphorylation.<ref>PMID:10449404</ref> <ref>PMID:11694597</ref> <ref>PMID:12529323</ref> 
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== Publication Abstract from PubMed ==
Clathrin-mediated endocytosis, the main trafficking route from the plasma membrane to the cytoplasm, is critical to many fundamental cellular processes. Clathrin, coupled to the membrane by adaptor proteins, is thought to play a major structural role in endocytosis by self-assembling into a cage-like lattice around the forming vesicle. Although clathrin adaptors are essential for endocytosis, little is known about their structural role in this process. Here we show that the membrane-binding domains of two conserved clathrin adaptors, Sla2 and Ent1, co-assemble in a PI(4,5)P2-dependent manner to form organized lattices on membranes. We determined the structure of the co-assembled lattice by electron cryo-microscopy and designed mutations that specifically impair the lattice formation in vitro. We show that these mutations block endocytosis in vivo. We suggest that clathrin adaptors not only link the polymerized clathrin to the membrane but also form an oligomeric structure, which is essential for membrane remodeling during endocytosis.

An Organized Co-assembly of Clathrin Adaptors Is Essential for Endocytosis.,Skruzny M, Desfosses A, Prinz S, Dodonova SO, Gieras A, Uetrecht C, Jakobi AJ, Abella M, Hagen WJ, Schulz J, Meijers R, Rybin V, Briggs JA, Sachse C, Kaksonen M Dev Cell. 2015 Apr 20;33(2):150-62. doi: 10.1016/j.devcel.2015.02.023. PMID:25898165<ref>PMID:25898165</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5ahv" style="background-color:#fffaf0;"></div>

==See Also==
*[[Epsin|Epsin]]
== References ==
<references/>
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</SX>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Abella M]]
[[Category: Briggs JAG]]
[[Category: Desfosses A]]
[[Category: Dodonova SO]]
[[Category: Gieras A]]
[[Category: Hagen WJH]]
[[Category: Jakobi AJ]]
[[Category: Kaksonen M]]
[[Category: Meijers R]]
[[Category: Prinz S]]
[[Category: Rybin V]]
[[Category: Sachse C]]
[[Category: Schulz J]]
[[Category: Skruzny M]]
[[Category: Uetrecht C]]