View source for 5a2g


==An esterase from anaerobic Clostridium hathewayi can hydrolyze aliphatic aromatic polyesters==
<StructureSection load='5a2g' size='340' side='right'caption='[[5a2g]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5a2g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Hungatella_hathewayi_DSM_13479 Hungatella hathewayi DSM 13479]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A2G FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.899&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a2g OCA], [https://pdbe.org/5a2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a2g RCSB], [https://www.ebi.ac.uk/pdbsum/5a2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a2g ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D3AU79_9CLOT D3AU79_9CLOT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Recently, a variety of biodegradable polymers have been developed as alternatives to recalcitrant materials. Although many studies on polyester biodegradability have focused on aerobic environments, there is much less known on biodegradation of polyesters in natural and artificial anaerobic habitats. Consequently, the potential of anaerobic biogas sludge to hydrolyze the synthetic compostable polyester PBAT (poly(butylene adipate-co-butylene terephthalate) was evaluated in this study. On the basis of reverse-phase high-performance liquid chromatography (RP-HPLC) analysis, accumulation of terephthalic acid (Ta) was observed in all anaerobic batches within the first 14 days. Thereafter, a decline of Ta was observed, which occurred presumably due to consumption by the microbial population. The esterase Chath_Est1 from the anaerobic risk 1 strain Clostridium hathewayi DSM-13479 was found to hydrolyze PBAT. Detailed characterization of this esterase including elucidation of the crystal structure was performed. The crystal structure indicates that Chath_Est1 belongs to the alpha/beta-hydrolases family. This study gives a clear hint that also micro-organisms in anaerobic habitats can degrade manmade PBAT.

An Esterase from Anaerobic Clostridium hathewayi Can Hydrolyze Aliphatic-Aromatic Polyesters.,Perz V, Hromic A, Baumschlager A, Steinkellner G, Pavkov-Keller T, Gruber K, Bleymaier K, Zitzenbacher S, Zankel A, Mayrhofer C, Sinkel C, Kueper U, Schlegel K, Ribitsch D, Guebitz GM Environ Sci Technol. 2016 Feb 29. PMID:26878094<ref>PMID:26878094</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5a2g" style="background-color:#fffaf0;"></div>

==See Also==
*[[Carboxylesterase 3D structures|Carboxylesterase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Hungatella hathewayi DSM 13479]]
[[Category: Large Structures]]
[[Category: Baumschlager A]]
[[Category: Bleymaier K]]
[[Category: Gruber K]]
[[Category: Guebitz GM]]
[[Category: Hromic A]]
[[Category: Kueper U]]
[[Category: Mayrhofer C]]
[[Category: Pavkov Keller T]]
[[Category: Perz V]]
[[Category: Ribitsch D]]
[[Category: Schlegel KA]]
[[Category: Sinkel C]]
[[Category: Steinkellner G]]
[[Category: Zankel A]]
[[Category: Zitzenbacher S]]