View source for 4xgz


==Crystal structure of human paxillin LD2 motif in complex with Fab fragment==
<StructureSection load='4xgz' size='340' side='right'caption='[[4xgz]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4xgz]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XGZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgz OCA], [https://pdbe.org/4xgz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xgz RCSB], [https://www.ebi.ac.uk/pdbsum/4xgz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xgz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PAXI_HUMAN PAXI_HUMAN] Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Focal adhesion protein paxillin links integrin and growth factor signaling to actin cytoskeleton. Most of paxillin signaling activity is regulated via leucine-rich LD motifs (LD1-LD5) located at the N-terminus. Here, we demonstrate a method to engineer highly selective synthetic antibodies (sABs) against LD2 and LD4 which are binding sites for focal adhesion kinase (FAK) and other proteins. Phage display selections against peptides were used to generate sABs recognizing each LD motif. In the obtained X-ray crystal structures of the LD-sAB complexes the LD motifs are helical and bind sABs through a hydrophobic side, similarly as in the structures with natural paxillin partners. The sABs are capable of pulling down endogenous paxillin in complex with FAK and can visualize paxillin in focal adhesions in cells. They were also used as selective inhibitors to effectively compete with focal adhesion targeting domain of FAK for the binding to LD2 and LD4. The sABs are tools for investigation of paxillin LD binding "platforms" and are capable of inhibiting paxillin interactions; and thereby useful as potential therapeutics in the future.

Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin.,Nocula-Lugowska M, Lugowski M, Salgia R, Kossiakoff AA J Mol Biol. 2015 Jun 15. pii: S0022-2836(15)00344-7. doi:, 10.1016/j.jmb.2015.06.004. PMID:26087144<ref>PMID:26087144</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4xgz" style="background-color:#fffaf0;"></div>

==See Also==
*[[Paxillin|Paxillin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Kossiakoff AA]]
[[Category: Lugowski M]]
[[Category: Nocula-Lugowska M]]
[[Category: Salgia R]]