View source for 4wt8


==Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex==
<StructureSection load='4wt8' size='340' side='right'caption='[[4wt8]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4wt8]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4wus 4wus], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4wwe 4wwe] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4wwt 4wwt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WT8 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3V6:BACTOBOLIN+A'>3V6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wt8 OCA], [https://pdbe.org/4wt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wt8 RCSB], [https://www.ebi.ac.uk/pdbsum/4wt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wt8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-A-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations.

Bactobolin A Binds to a Site on the 70S Ribosome Distinct from Previously Seen Antibiotics.,Amunts A, Fiedorczuk K, Truong TT, Chandler J, Peter Greenberg E, Ramakrishnan V J Mol Biol. 2015 Jan 3. pii: S0022-2836(14)00654-8. doi:, 10.1016/j.jmb.2014.12.018. PMID:25562208<ref>PMID:25562208</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4wt8" style="background-color:#fffaf0;"></div>

==See Also==
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB8]]
[[Category: Amunts A]]
[[Category: Fiedorczuk K]]
[[Category: Ramakrishnan V]]