View source for 4wm9


==Acinetobacter baumanii OXA-24 complex with Avibactam==
<StructureSection load='4wm9' size='340' side='right'caption='[[4wm9]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4wm9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WM9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NXL:(2S,5R)-1-FORMYL-5-[(SULFOOXY)AMINO]PIPERIDINE-2-CARBOXAMIDE'>NXL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wm9 OCA], [https://pdbe.org/4wm9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wm9 RCSB], [https://www.ebi.ac.uk/pdbsum/4wm9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wm9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8RLA6_ACIBA Q8RLA6_ACIBA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Class D (or OXA-type) beta-lactamases have expanded to be the most diverse group of serine beta-lactamases with a highly heterogeneous beta-lactam hydrolysis profile and are typically resistant to marketed beta-lactamase inhibitors. Class D enzymes are increasingly found in multi-drug resistant (MDR) Acinetobacter baumannii, Pseudomonas aeruginosa, and various species of the Enterobacteriaceae and are posing a serious threat to the clinical utility of beta-lactams including the carbapenems, which are typically reserved as the drugs of last resort. Avibactam, a novel non-beta-lactam beta-lactamase inhibitor, not only inhibits all class A and class C beta-lactamases, but also has the promise of inhibition of certain OXA enzymes, thus extending the antibacterial activity of the beta-lactam used in combination to the organisms that produce these enzymes. X-ray structures of OXA-24 and OXA-48 in complex with avibactam revealed the binding mode of this inhibitor in this diverse class of enzymes and provide a rationale for selective inhibition of OXA-48 members. Additionally, various subunits of the OXA-48 structure in the asymmetric unit provide snapshots of different states of the inhibited enzyme. Overall, these data provide the first structural evidence of the exceptionally slow reversibility observed with avibactam in class D beta-lactamases. Mechanisms for acylation and deacylation of avibactam by class D enzymes are proposed, and the likely extent of inhibition of class D beta-lactamases by avibactam is discussed.

Molecular basis of selective inhibition and slow reversibility of avibactam against Class D carbapenemases: a structure-guided study of OXA-24 and OXA-48.,Lahiri SD, Mangani S, Jahic H, Benvenuti M, Durand-Reville TF, De Luca F, Ehmann DE, Rossolini GM, Alm RA, Docquier JD ACS Chem Biol. 2014 Nov 19. PMID:25406838<ref>PMID:25406838</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4wm9" style="background-color:#fffaf0;"></div>

==See Also==
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Acinetobacter baumannii]]
[[Category: Large Structures]]
[[Category: Benvenuti M]]
[[Category: Docquier J-D]]
[[Category: Mangani S]]