View source for 4v67


==Crystal structure of a translation termination complex formed with release factor RF2.==
<StructureSection load='4v67' size='340' side='right'caption='[[4v67]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4v67]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3f1e 3f1e], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3f1f 3f1f], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3f1g 3f1g] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3f1h 3f1h]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V67 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v67 OCA], [https://pdbe.org/4v67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v67 RCSB], [https://www.ebi.ac.uk/pdbsum/4v67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v67 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS2_THET2 RS2_THET2] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report the crystal structure of a translation termination complex formed by the Thermus thermophilus 70S ribosome bound with release factor RF2, in response to a UAA stop codon, solved at 3 A resolution. The backbone of helix alpha5 and the side chain of serine of the conserved SPF motif of RF2 recognize U1 and A2 of the stop codon, respectively. A3 is unstacked from the first 2 bases, contacting Thr-216 and Val-203 of RF2 and stacking on G530 of 16S rRNA. The structure of the RF2 complex supports our previous proposal that conformational changes in the ribosome in response to recognition of the stop codon stabilize rearrangement of the switch loop of the release factor, resulting in docking of the universally conserved GGQ motif in the PTC of the 50S subunit. As seen for the RF1 complex, the main-chain amide nitrogen of glutamine in the GGQ motif is positioned to contribute directly to catalysis of peptidyl-tRNA hydrolysis, consistent with mutational studies, which show that most side-chain substitutions of the conserved glutamine have little effect. We show that when the H-bonding capability of the main-chain N-H of the conserved glutamine is eliminated by substitution with proline, peptidyl-tRNA esterase activity is abolished, consistent with its proposed role in catalysis.

Crystal structure of a translation termination complex formed with release factor RF2.,Korostelev A, Asahara H, Lancaster L, Laurberg M, Hirschi A, Zhu J, Trakhanov S, Scott WG, Noller HF Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19684-9. Epub 2008 Dec 8. PMID:19064930<ref>PMID:19064930</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4v67" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Asahara H]]
[[Category: Hirschi A]]
[[Category: Korostelev A]]
[[Category: Lancaster L]]
[[Category: Laurberg M]]
[[Category: Noller HF]]