View source for 4v4h


==Crystal structure of the bacterial ribosome from Escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution.==
<StructureSection load='4v4h' size='340' side='right'caption='[[4v4h]], [[Resolution|resolution]] 3.46&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4v4h]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vs5 1vs5], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vs6 1vs6], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vs7 1vs7] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vs8 1vs8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V4H FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.46&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KSG:(1S,2R,3S,4R,5S,6S)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL+2-AMINO-4-{[CARBOXY(IMINO)METHYL]AMINO}-2,3,4,6-TETRADEOXY-ALPHA-D-ARABINO-HEXOPYRANOSIDE'>KSG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4h OCA], [https://pdbe.org/4v4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v4h RCSB], [https://www.ebi.ac.uk/pdbsum/4v4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v4h ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS6_ECOLI RS6_ECOLI] Binds together with S18 to 16S ribosomal RNA.[HAMAP-Rule:MF_00360]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The prokaryotic ribosome is an important target of antibiotic action. We determined the X-ray structure of the aminoglycoside kasugamycin (Ksg) in complex with the Escherichia coli 70S ribosome at 3.5-A resolution. The structure reveals that the drug binds within the messenger RNA channel of the 30S subunit between the universally conserved G926 and A794 nucleotides in 16S ribosomal RNA, which are sites of Ksg resistance. To our surprise, Ksg resistance mutations do not inhibit binding of the drug to the ribosome. The present structural and biochemical results indicate that inhibition by Ksg and Ksg resistance are closely linked to the structure of the mRNA at the junction of the peptidyl-tRNA and exit-tRNA sites (P and E sites).

Structural analysis of kasugamycin inhibition of translation.,Schuwirth BS, Day JM, Hau CW, Janssen GR, Dahlberg AE, Cate JH, Vila-Sanjurjo A Nat Struct Mol Biol. 2006 Oct;13(10):879-86. Epub 2006 Sep 24. PMID:16998486<ref>PMID:16998486</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4v4h" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Cate JHD]]
[[Category: Schuwirth BS]]
[[Category: Vila-Sanjurjo A]]