View source for 4ujc


==mammalian 80S HCV-IRES initiation complex with eIF5B POST-like state==
<SX load='4ujc' size='340' side='right' viewer='molstar' caption='[[4ujc]], [[Resolution|resolution]] 9.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ujc]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4upz 4upz], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4uq0 4uq0], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4uq1 4uq1] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4uq4 4uq4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UJC FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ujc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ujc OCA], [https://pdbe.org/4ujc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ujc RCSB], [https://www.ebi.ac.uk/pdbsum/4ujc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ujc ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The universally conserved eukaryotic initiation factor (eIF) 5B, a translational GTPase, is essential for canonical translation initiation. It is also required for initiation facilitated by the internal ribosomal entry site (IRES) of hepatitis C virus (HCV) RNA. eIF5B promotes joining of 60S ribosomal subunits to 40S ribosomal subunits bound by initiator tRNA (Met-tRNAiMet). However, the exact molecular mechanism by which eIF5B acts has not been established. Here we present cryo-EM reconstructions of the mammalian 80S-HCV-IRES-Met-tRNAiMet-eIF5B-GMPPNP complex. We obtained two substates distinguished by the rotational state of the ribosomal subunits and the configuration of initiator tRNA in the peptidyl (P) site. Accordingly, a combination of conformational changes in the 80S ribosome and in initiator tRNA facilitates binding of the Met-tRNAiMet to the 60S P site and redefines the role of eIF5B as a tRNA-reorientation factor.

Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV-IRES RNA.,Yamamoto H, Unbehaun A, Loerke J, Behrmann E, Collier M, Burger J, Mielke T, Spahn CM Nat Struct Mol Biol. 2014 Aug;21(8):721-727. doi: 10.1038/nsmb.2859. Epub 2014, Jul 27. PMID:25064512<ref>PMID:25064512</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4ujc" style="background-color:#fffaf0;"></div>

==See Also==
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
*[[3D sructureseceptor for activated protein kinase C 1|3D sructureseceptor for activated protein kinase C 1]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Behrmann E]]
[[Category: Burger J]]
[[Category: Loerke J]]
[[Category: Marianne C]]
[[Category: Mielke T]]
[[Category: Spahn CMT]]
[[Category: Unbehaun A]]
[[Category: Yamamoto H]]