View source for 4u8h


==Crystal Structure of Mammalian Period-Cryptochrome Complex==
<StructureSection load='4u8h' size='340' side='right'caption='[[4u8h]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4u8h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U8H FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.798&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u8h OCA], [https://pdbe.org/4u8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u8h RCSB], [https://www.ebi.ac.uk/pdbsum/4u8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u8h ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CRY2_MOUSE CRY2_MOUSE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The mammalian circadian clock is driven by a transcriptional-translational feedback loop, which produces robust 24-hr rhythms. Proper oscillation of the clock depends on the complex formation and periodic turnover of the Period and Cryptochrome proteins, which together inhibit their own transcriptional activator complex, CLOCK-BMAL1. We determined the crystal structure of the CRY-binding domain (CBD) of PER2 in complex with CRY2 at 2.8 A resolution. PER2-CBD adopts a highly extended conformation, embracing CRY2 with a sinuous binding mode. Its N-terminal end tucks into CRY adjacent to a large pocket critical for CLOCK-BMAL1 binding, while its C-terminal half flanks the CRY2 C-terminal helix and sterically hinders the recognition of CRY2 by the FBXL3 ubiquitin ligase. Unexpectedly, a strictly conserved intermolecular zinc finger, whose integrity is important for clock rhythmicity, further stabilizes the complex. Our structure-guided analyses show that these interspersed CRY-interacting regions represent multiple functional modules of PERs at the CRY-binding interface.DOI: http://dx.doi.org/10.7554/eLife.03674.001.

Molecular assembly of the period-cryptochrome circadian transcriptional repressor complex.,Nangle SN, Rosensweig C, Koike N, Tei H, Takahashi JS, Green CB, Zheng N Elife. 2014 Aug 15;3:e03674. doi: 10.7554/eLife.03674. PMID:25127877<ref>PMID:25127877</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4u8h" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cryptochrome 3D structures|Cryptochrome 3D structures]]
*[[Period circadian protein|Period circadian protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Green CB]]
[[Category: Koike N]]
[[Category: Nangle SN]]
[[Category: Rosensweig C]]
[[Category: Takahashi JS]]
[[Category: Tei H]]
[[Category: Zheng N]]