View source for 4rs3


==Crystal structure of carbohydrate transporter A0QYB3 from Mycobacterium smegmatis str. MC2 155, target EFI-510969, in complex with xylitol==
<StructureSection load='4rs3' size='340' side='right'caption='[[4rs3]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4rs3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RS3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RS3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=XYL:D-XYLITOL'>XYL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rs3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rs3 OCA], [https://pdbe.org/4rs3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rs3 RCSB], [https://www.ebi.ac.uk/pdbsum/4rs3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rs3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/XYPA_MYCS2 XYPA_MYCS2] Part of an ABC transporter complex likely involved in xylitol import. Binds xylitol.<ref>PMID:26560079</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We describe a general integrated bioinformatic and experimental strategy to discover the in vitro enzymatic activities and in vivo functions (metabolic pathways) of uncharacterized enzymes discovered in microbial genome projects using the ligand specificities of the solute binding proteins (SBPs) for ABC transporters. Using differential scanning fluorimetry, we determined that the SBP for an ABC transporter encoded by the genome of Mycobacterium smegmatis is stabilized by d-threitol. Using sequence similarity networks and genome neighborhood networks to guide selection of target proteins for pathway enzymes, we applied both in vitro and in vivo experimental approaches to discover novel pathways for catabolism of d-threitol, l-threitol, and erythritol.

A General Strategy for the Discovery of Metabolic Pathways: d-Threitol, l-Threitol, and Erythritol Utilization in Mycobacterium smegmatis.,Huang H, Carter MS, Vetting MW, Al-Obaidi N, Patskovsky Y, Almo SC, Gerlt JA J Am Chem Soc. 2015 Nov 25;137(46):14570-3. doi: 10.1021/jacs.5b08968. Epub 2015 , Nov 12. PMID:26560079<ref>PMID:26560079</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4rs3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycolicibacterium smegmatis MC2 155]]
[[Category: Al Obaidi N]]
[[Category: Almo SC]]
[[Category: Attonito JD]]
[[Category: Bhosle R]]
[[Category: Chamala S]]
[[Category: Chowdhury S]]
[[Category: Gerlt JA]]
[[Category: Hillerich B]]
[[Category: Lafleur J]]
[[Category: Love J]]
[[Category: Morisco LL]]
[[Category: Patskovsky Y]]
[[Category: Scott Glenn A]]
[[Category: Siedel RD]]
[[Category: Toro R]]
[[Category: Wasserman SR]]
[[Category: Whalen KL]]