View source for 4piv


==Human Fatty Acid Synthase Psi/KR Tri-Domain with NADPH and GSK2194069==
<StructureSection load='4piv' size='340' side='right'caption='[[4piv]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4piv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PIV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.299&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2W4:4-[4-(1-BENZOFURAN-5-YL)PHENYL]-5-{[(3S)-1-(CYCLOPROPYLCARBONYL)PYRROLIDIN-3-YL]METHYL}-2,4-DIHYDRO-3H-1,2,4-TRIAZOL-3-ONE'>2W4</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4piv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4piv OCA], [https://pdbe.org/4piv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4piv RCSB], [https://www.ebi.ac.uk/pdbsum/4piv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4piv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FAS_HUMAN FAS_HUMAN] Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
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== Publication Abstract from PubMed ==
Human fatty acid synthase (hFAS) is a complex, multifunctional enzyme that is solely responsible for the de novo synthesis of long chain fatty acids. hFAS is highly expressed in a number of cancers, with low expression observed in most normal tissues. Although normal tissues tend to obtain fatty acids from the diet, tumor tissues rely on de novo fatty acid synthesis, making hFAS an attractive metabolic target for the treatment of cancer. We describe here the identification of GSK2194069, a potent and specific inhibitor of the beta-ketoacyl reductase (KR) activity of hFAS; the characterization of its enzymatic and cellular mechanism of action; and its inhibition of human tumor cell growth. We also present the design of a new protein construct suitable for crystallography, which resulted in what is to our knowledge the first co-crystal structure of the human KR domain and includes a bound inhibitor.

A human fatty acid synthase inhibitor binds beta-ketoacyl reductase in the keto-substrate site.,Hardwicke MA, Rendina AR, Williams SP, Moore ML, Wang L, Krueger JA, Plant RN, Totoritis RD, Zhang G, Briand J, Burkhart WA, Brown KK, Parrish CA Nat Chem Biol. 2014 Sep;10(9):774-9. doi: 10.1038/nchembio.1603. Epub 2014 Aug 3. PMID:25086508<ref>PMID:25086508</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4piv" style="background-color:#fffaf0;"></div>

==See Also==
*[[Fatty acid synthase 3D structures|Fatty acid synthase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Brown KK]]
[[Category: Hardwicke MA]]
[[Category: Parrish CA]]
[[Category: Wang L]]
[[Category: Williams SP]]