View source for 4pbw


==Crystal structure of chicken receptor protein tyrosine phosphatase sigma in complex with TrkC==
<StructureSection load='4pbw' size='340' side='right'caption='[[4pbw]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4pbw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PBW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pbw OCA], [https://pdbe.org/4pbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pbw RCSB], [https://www.ebi.ac.uk/pdbsum/4pbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pbw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NTRK3_CHICK NTRK3_CHICK] Receptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase and PLCG1. The KT and KD isoforms fail to stimulate transformation, process outgrowth or survival. Isoform KI25 exhibits tyrosine phosphorylation in the absence of ligand and is unable to mediate survival of neuronal cells.
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== Publication Abstract from PubMed ==
Receptor protein tyrosine phosphatase sigma (RPTPsigma) regulates neuronal extension and acts as a presynaptic nexus for multiple protein and proteoglycan interactions during synaptogenesis. Unknown mechanisms govern the shift in RPTPsigma function, from outgrowth promotion to synaptic organization. Here, we report crystallographic, electron microscopic and small-angle X-ray scattering analyses, which reveal sufficient inter-domain flexibility in the RPTPsigma extracellular region for interaction with both cis (same cell) and trans (opposite cell) ligands. Crystal structures of RPTPsigma bound to its postsynaptic ligand TrkC detail an interaction surface partially overlapping the glycosaminoglycan-binding site. Accordingly, heparan sulphate and heparin oligomers compete with TrkC for RPTPsigma binding in vitro and disrupt TrkC-dependent synaptic differentiation in neuronal co-culture assays. We propose that transient RPTPsigma ectodomain emergence from the presynaptic proteoglycan layer allows capture by TrkC to form a trans-synaptic complex, the consequent reduction in RPTPsigma flexibility potentiating interactions with additional ligands to orchestrate excitatory synapse formation.

Structural basis for extracellular cis and trans RPTPsigma signal competition in synaptogenesis.,Coles CH, Mitakidis N, Zhang P, Elegheert J, Lu W, Stoker AW, Nakagawa T, Craig AM, Jones EY, Aricescu AR Nat Commun. 2014 Nov 11;5:5209. doi: 10.1038/ncomms6209. PMID:25385546<ref>PMID:25385546</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4pbw" style="background-color:#fffaf0;"></div>

==See Also==
*[[TrkB tyrosine kinase receptor|TrkB tyrosine kinase receptor]]
*[[Tyrosine kinase receptor|Tyrosine kinase receptor]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gallus gallus]]
[[Category: Large Structures]]
[[Category: Aricescu AR]]
[[Category: Coles CH]]
[[Category: Craig AM]]
[[Category: Elegheert J]]
[[Category: Jones EY]]
[[Category: Lu W]]
[[Category: Mitakidis N]]
[[Category: Nakagawa T]]
[[Category: Stoker AW]]
[[Category: Zhang P]]