View source for 4ke7


==Crystal structure of Monoglyceride lipase from Bacillus sp. H257 in complex with an 1-myristoyl glycerol analogue==
<StructureSection load='4ke7' size='340' side='right'caption='[[4ke7]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ke7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._H-257 Bacillus sp. H-257]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KE7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KE7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.699&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1QX:DODECYL+HYDROGEN+(S)-(3-AZIDOPROPYL)PHOSPHONATE'>1QX</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ke7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ke7 OCA], [https://pdbe.org/4ke7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ke7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ke7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ke7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MGLP_BAC25 MGLP_BAC25] Hydrolyzes monoacylglycerols, with the highest activity occurring with 1-monolauroylglycerol.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Monoacylglycerol lipases (MGLs) play an important role in lipid catabolism across all kingdoms of life by catalyzing the release of free fatty acids from monoacylglycerols. The three-dimensional structures of human and a bacterial MGL were determined only recently as the first members of this lipase family. In addition to the alpha/beta hydrolase core, they showed unexpected structural similarities even in the cap region. Nevertheless, the structural basis for substrate binding and conformational changes of MGLs is poorly understood. Here we present a comprehensive study of five crystal structures of MGL from Bacillus sp. H257 in its free form and in complex with different substrate analogues and the natural substrate 1-lauroyl glycerol (1-LG). The occurrence of different conformations reveals a high degree of conformational plasticity of the cap region. We identify a specific residue, Ile145 that might act as a gate keeper restricting access to the binding site. Site directed mutagenesis of Ile145 leads to significantly reduced hydrolase activity. Bacterial MGL in complex with 1-LG, myristoyl-, palmitoyl and stearoyl-substrate analogues enable identification of the binding sites for the alkyl chain and the glycerol moiety of the natural ligand. They also provide snapshots of the hydrolytic reaction of bMGL at different stages. The alkyl chains are buried in a hydrophobic tunnel in an extended conformation. Binding of the glycerol moiety is mediated via Glu156 and water molecules. Analysis of the structural features responsible for cap plasticity and the binding modes of the ligands suggest conservation of these features also in human MGL.

Conformational plasticity and ligand binding of bacterial monoacylglycerol lipase.,Rengachari S, Aschauer P, Schittmayer M, Mayer N, Gruber K, Breinbauer R, Birner-Gruenberger R, Dreveny I, Oberer M J Biol Chem. 2013 Sep 6. PMID:24014019<ref>PMID:24014019</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ke7" style="background-color:#fffaf0;"></div>

==See Also==
*[[Lipase 3D Structures|Lipase 3D Structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus sp. H-257]]
[[Category: Large Structures]]
[[Category: Aschauer P]]
[[Category: Dreveny I]]
[[Category: Gruber K]]
[[Category: Oberer M]]
[[Category: Rengachari S]]