View source for 4jly


==Dodecameric structure of spermidine N-acetyltransferase from Vibrio cholerae==
<StructureSection load='4jly' size='340' side='right'caption='[[4jly]], [[Resolution|resolution]] 2.88&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4jly]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JLY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JLY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.882&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jly OCA], [https://pdbe.org/4jly PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jly RCSB], [https://www.ebi.ac.uk/pdbsum/4jly PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jly ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ATDA_VIBCH ATDA_VIBCH] Involved in the protection against polyamine toxicity by regulating their concentration. Catalyzes the transfer of an acetyl group from acetyl coenzyme A (AcCoA) to the primary amino groups of spermidine to yield N(1)- and N(8)-acetylspermidine. It can use polyamines such as spermine and N(1)-acetylspermine, but not putrescine or cadaverine.<ref>PMID:23184347</ref> <ref>PMID:25623305</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The spermidine N-acetyltransferase SpeG is a dodecameric enzyme that catalyzes the transfer of an acetyl group from acetyl-coenzyme A to polyamines such as spermidine and spermine. SpeG has an allosteric polyamine-binding site and acetylating polyamines regulates their intracellular concentrations. The structures of SpeG from Vibrio cholerae in complexes with polyamines and cofactor have been characterized earlier. Here, we present the dodecameric structure of SpeG from V. cholerae in a ligand-free form in three different conformational states: open, intermediate and closed. All structures were crystallized in C2 space group symmetry and contain 6 monomers in the asymmetric unit cell. Two hexamers related by crystallographic twofold symmetry form the SpeG dodecamer. The open and intermediate states have a unique open dodecameric ring. This SpeG dodecamer is asymmetric except for the one twofold axis and is unlike any known dodecameric structure. Using a fluorescence thermal shift assay, size exclusion chromatography with multi-angle light scattering, small angle X-ray scattering analysis, negative stain electron microscopy, and structural analysis we demonstrate that this unique open dodecameric state exists in solution. Our combined results indicate that polyamines trigger conformational changes and induce the symmetric closed dodecameric state of the protein when they bind to their allosteric sites.

Substrate induced allosteric change in the quaternary structure of the spermidine N-acetyltransferase SpeG.,Filippova EV, Weigand S, Osipiuk J, Kiryukhina O, Joachimiak A, Anderson WF J Mol Biol. 2015 Sep 24. pii: S0022-2836(15)00536-7. doi:, 10.1016/j.jmb.2015.09.013. PMID:26410587<ref>PMID:26410587</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4jly" style="background-color:#fffaf0;"></div>

==See Also==
*[[Spermidine/spermine N-acetyltransferase|Spermidine/spermine N-acetyltransferase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
[[Category: Anderson WF]]
[[Category: Filippova EV]]
[[Category: Kiryukhina O]]
[[Category: Kuhn ML]]
[[Category: Minasov G]]
[[Category: Shuvalova L]]