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==Structure of the tethered N-terminus of Alzheimer's disease A peptide==
<StructureSection load='4f37' size='340' side='right'caption='[[4f37]], [[Resolution|resolution]] 2.57&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4f37]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F37 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f37 OCA], [https://pdbe.org/4f37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f37 RCSB], [https://www.ebi.ac.uk/pdbsum/4f37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f37 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.
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== Publication Abstract from PubMed ==
Alzheimer's disease is the most common form of dementia in humans and is related to the accumulation of the amyloid-beta (Abeta) peptide and its interaction with metals (Cu, Fe and Zn) in the brain. Crystallographic structural information about Abeta peptide deposits and the details of the metal binding site is limited due to the heterogeneous nature of aggregation states formed by the peptide. Here we present a crystal structure of Abeta residues 1-16 fused to the N-terminus of the E. coli immunity protein Im7, and stabilized with the Fab fragment of the anti-Abeta N-terminal antibody WO2. The structure demonstrates that Abeta residues 10-16, which are not in complex with the antibody, adopt a mixture of local polyproline II (PPII) helix and turn type conformations, enhancing co-operativity between the two adjacent histidine residues His13 and His14. Furthermore, this relatively rigid region of Abeta (residues 10-16) appear as an almost independent unit available for trapping metal ions and provides a rationale for the His13-metal-His14 coordination in the Abeta1-16 fragment implicated in Abeta metal binding. This novel structure therefore has the potential to provide a foundation for investigating the effect of metal ion binding to Abeta and illustrates a potential target for development of future Alzheimer's disease therapeutics aimed at stabilizing the N-terminal monomer structure, in particular residues His13 and His14, and preventing Abeta metal binding-induced neurotoxicity. (c) Proteins 2013;. (c) 2013 Wiley Periodicals, Inc.

Structural studies of the tethered N-terminus of the Alzheimer's disease Abeta peptide.,Nisbet RM, Nuttall SD, Robert R, Caine JM, Dolezal O, Hattarki M, Pearce LA, Davydova N, Masters CL, Varghese JN, Streltsov VA Proteins. 2013 Apr 23. doi: 10.1002/prot.24312. PMID:23609990<ref>PMID:23609990</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4f37" style="background-color:#fffaf0;"></div>

==See Also==
*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Caine JM]]
[[Category: Davydova N]]
[[Category: Hittaki M]]
[[Category: Masters CL]]
[[Category: Nisbet RM]]
[[Category: Nuttall SD]]
[[Category: Pearce LA]]
[[Category: Rober R]]
[[Category: Streltsov VA]]
[[Category: Varghese JN]]