View source for 4dns


==Crystal structure of Bermuda grass isoallergen BG60 provides insight into the various cross-allergenicity of the pollen group 4 allergens==
<StructureSection load='4dns' size='340' side='right'caption='[[4dns]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4dns]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cynodon_dactylon Cynodon dactylon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DNS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dns OCA], [https://pdbe.org/4dns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dns RCSB], [https://www.ebi.ac.uk/pdbsum/4dns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dns ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CYND4_CYNDA CYND4_CYNDA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of Cyn d 4, the group 4 allergen from Bermuda grass, is reported at 2.15 A resolution and is the first crystal structure of a naturally isolated pollen allergen. A conserved N-terminal segment that is only present in the large isoallergens forms extensive interactions with surrounding residues and hence greatly enhances the structural stability of the protein. Cyn d 4 contains an FAD cofactor that is covalently linked to His88 and Cys152. To date, all identified bicovalent flavoproteins are oxidases and their substrates are either sugars or secondary metabolites. A deep large hydrophobic substrate-binding cleft is present. Thus, Cyn d 4 may be an oxidase that is involved in the biosynthesis of a pollen-specific metabolite. Cyn d 4 shares ~70% sequence identity with the Pooideae group 4 allergens. Various cross-reactivities between grass pollen group 4 allergens have previously been demonstrated using sera from allergic patients. The protein surface displays an unusually large number of positively charged clusters, reflecting the high pI of ~10. 38 decapeptides that cover the solvent-accessible sequences did not show any significant IgE-binding activity using sera with high Cyn d 4 reactivity from four patients, suggesting that the IgE epitopes of Cyn d 4 are predominantly conformational in nature. Several group 4 structures were then modelled and their potential cross-reactive and species-specific IgE epitopes were proposed.

Various cross-reactivity of the grass pollen group 4 allergens: crystallographic study of the Bermuda grass isoallergen Cyn d 4.,Huang TH, Peng HJ, Su SN, Liaw SH Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1303-10. Epub 2012 Sep, 13. PMID:22993084<ref>PMID:22993084</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4dns" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cynodon dactylon]]
[[Category: Large Structures]]
[[Category: Huang TH]]
[[Category: Liaw SH]]
[[Category: Peng HJ]]
[[Category: Su SN]]