View source for 4bbv


==The PB0 Photocycle Intermediate of Photoactive Yellow Protein==
<StructureSection load='4bbv' size='340' side='right'caption='[[4bbv]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4bbv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dsm_244 Dsm 244]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BBV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BBV FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d7e|1d7e]], [[1f98|1f98]], [[1f9i|1f9i]], [[1gsv|1gsv]], [[1gsw|1gsw]], [[1gsx|1gsx]], [[1kou|1kou]], [[1nwz|1nwz]], [[1odv|1odv]], [[1ot6|1ot6]], [[1ot9|1ot9]], [[1ota|1ota]], [[1otb|1otb]], [[1otd|1otd]], [[1ote|1ote]], [[1oti|1oti]], [[1s1y|1s1y]], [[1s1z|1s1z]], [[1s4r|1s4r]], [[1s4s|1s4s]], [[1t18|1t18]], [[1t19|1t19]], [[1t1a|1t1a]], [[1t1b|1t1b]], [[1t1c|1t1c]], [[1ts0|1ts0]], [[1ts6|1ts6]], [[1ts7|1ts7]], [[1ts8|1ts8]], [[1ugu|1ugu]], [[1uwn|1uwn]], [[1uwp|1uwp]], [[1xfn|1xfn]], [[1xfq|1xfq]], [[2d01|2d01]], [[2d02|2d02]], [[2phy|2phy]], [[2pyp|2pyp]], [[2pyr|2pyr]], [[3phy|3phy]], [[3pyp|3pyp]], [[4b9o|4b9o]], [[4bbt|4bbt]], [[4bbu|4bbu]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bbv OCA], [https://pdbe.org/4bbv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bbv RCSB], [https://www.ebi.ac.uk/pdbsum/4bbv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bbv ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA]] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response. 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To understand how signaling proteins function, it is crucial to know the time-ordered sequence of events that lead to the signaling state. We recently developed on the BioCARS 14-IDB beamline at the Advanced Photon Source the infrastructure required to characterize structural changes in protein crystals with near-atomic spatial resolution and 150-ps time resolution, and have used this capability to track the reversible photocycle of photoactive yellow protein (PYP) following trans-to-cis photoisomerization of its p-coumaric acid (pCA) chromophore over 10 decades of time. The first of four major intermediates characterized in this study is highly contorted, with the pCA carbonyl rotated nearly 90 degrees out of the plane of the phenolate. A hydrogen bond between the pCA carbonyl and the Cys69 backbone constrains the chromophore in this unusual twisted conformation. Density functional theory calculations confirm that this structure is chemically plausible and corresponds to a strained cis intermediate. This unique structure is short-lived ( approximately 600 ps), has not been observed in prior cryocrystallography experiments, and is the progenitor of intermediates characterized in previous nanosecond time-resolved Laue crystallography studies. The structural transitions unveiled during the PYP photocycle include trans/cis isomerization, the breaking and making of hydrogen bonds, formation/relaxation of strain, and gated water penetration into the interior of the protein. This mechanistically detailed, near-atomic resolution description of the complete PYP photocycle provides a framework for understanding signal transduction in proteins, and for assessing and validating theoretical/computational approaches in protein biophysics.

Watching a signaling protein function in real time via 100-ps time-resolved Laue crystallography.,Schotte F, Cho HS, Kaila VR, Kamikubo H, Dashdorj N, Henry ER, Graber TJ, Henning R, Wulff M, Hummer G, Kataoka M, Anfinrud PA Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19256-61. doi:, 10.1073/pnas.1210938109. Epub 2012 Nov 6. PMID:23132943<ref>PMID:23132943</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4bbv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Dsm 244]]
[[Category: Large Structures]]
[[Category: Phototropin]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Anfinrud, P A]]
[[Category: Cho, H S]]
[[Category: Dashdorj, N]]
[[Category: Graber, T J]]
[[Category: Henning, R]]
[[Category: Henry, E R]]
[[Category: Hummer, G]]
[[Category: Kaila, V R.I]]
[[Category: Kamikubo, H]]
[[Category: Kataoka, M]]
[[Category: Schotte, F]]
[[Category: Wulff, M]]
[[Category: Chromophore]]
[[Category: Light sensor]]
[[Category: Lov]]
[[Category: Pa]]
[[Category: Photoreceptor]]
[[Category: Photoreceptor protein]]
[[Category: Receptor]]
[[Category: Sensory transduction]]
[[Category: Signaling protein]]