View source for 4b3k


==Family 1 6-phospho-beta-D glycosidase from Streptococcus pyogenes==
<StructureSection load='4b3k' size='340' side='right'caption='[[4b3k]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4b3k]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B3K FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3k OCA], [https://pdbe.org/4b3k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b3k RCSB], [https://www.ebi.ac.uk/pdbsum/4b3k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b3k ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q99YP9_STRP1 Q99YP9_STRP1] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The group A streptococcus Streptococcus pyogenes is the causative agent of a wide spectrum of invasive infections, including necrotizing fasciitis, scarlet fever and toxic shock syndrome. In the context of its carbohydrate chemistry, it is interesting that S. pyogenes (in this work strain M1 GAS SF370) displays a spectrum of oligosaccharide-processing enzymes that are located in close proximity on the genome but that the in vivo function of these proteins remains unknown. These proteins include different sugar transporters (SPy1593 and SPy1595), both GH125 alpha-1,6- and GH38 alpha-1,3-mannosidases (SPy1603 and SPy1604), a GH84 beta-hexosaminidase (SPy1600) and a putative GH2 beta-galactosidase (SPy1586), as well as SPy1599, a family GH1 `putative beta-glucosidase'. Here, the solution of the three-dimensional structure of SPy1599 in a number of crystal forms complicated by unusual crystallographic twinning is reported. The structure is a classical (beta/alpha)(8)-barrel, consistent with CAZy family GH1 and other members of the GH-A clan. SPy1599 has been annotated in sequence depositions as a beta-glucosidase (EC 3.2.1.21), but no such activity could be found; instead, three-dimensional structural overlaps with other enzymes of known function suggested that SPy1599 contains a phosphate-binding pocket in the active site and has possible 6-phospho-beta-glycosidase activity. Subsequent kinetic analysis indeed showed that SPy1599 has 6-phospho-beta-glucosidase (EC 3.2.1.86) activity. These data suggest that SPy1599 is involved in the intracellular degradation of 6-phosphoglycosides, which are likely to originate from import through one of the organism's many phosphoenolpyruvate phosphotransfer systems (PEP-PTSs).

Structure and activity of the Streptococcus pyogenes family GH1 6-phospho-beta-glucosidase SPy1599.,Stepper J, Dabin J, Eklof JM, Thongpoo P, Kongsaeree P, Taylor EJ, Turkenburg JP, Brumer H, Davies GJ Acta Crystallogr D Biol Crystallogr. 2013 Jan;69(Pt 1):16-23. doi:, 10.1107/S0907444912041005. Epub 2012 Dec 20. PMID:23275159<ref>PMID:23275159</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4b3k" style="background-color:#fffaf0;"></div>

==See Also==
*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pyogenes]]
[[Category: Brumer H]]
[[Category: Dabin J]]
[[Category: Davies GJ]]
[[Category: Ekloef JM]]
[[Category: Kongsaeree PT]]
[[Category: Stepper J]]
[[Category: Taylor EJ]]
[[Category: Thongpoo P]]
[[Category: Turkenburg JP]]