View source for 4ad9


==Crystal structure of human LACTB2.==
<StructureSection load='4ad9' size='340' side='right'caption='[[4ad9]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ad9]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AD9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ad9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ad9 OCA], [https://pdbe.org/4ad9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ad9 RCSB], [https://www.ebi.ac.uk/pdbsum/4ad9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ad9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LACB2_HUMAN LACB2_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Post-transcriptional control of mitochondrial gene expression, including the processing and generation of mature transcripts as well as their degradation, is a key regulatory step in gene expression in human mitochondria. Consequently, identification of the proteins responsible for RNA processing and degradation in this organelle is of great importance. The metallo-beta-lactamase (MBL) is a candidate protein family that includes ribo- and deoxyribonucleases. In this study, we discovered a function for LACTB2, an orphan MBL protein found in mammalian mitochondria. Solving its crystal structure revealed almost perfect alignment of the MBL domain with CPSF73, as well as to other ribonucleases of the MBL superfamily. Recombinant human LACTB2 displayed robust endoribonuclease activity on ssRNA with a preference for cleavage after purine-pyrimidine sequences. Mutational analysis identified an extended RNA-binding site. Knockdown of LACTB2 in cultured cells caused a moderate but significant accumulation of many mitochondrial transcripts, and its overexpression led to the opposite effect. Furthermore, manipulation of LACTB2 expression resulted in cellular morphological deformation and cell death. Together, this study discovered that LACTB2 is an endoribonuclease that is involved in the turnover of mitochondrial RNA, and is essential for mitochondrial function in human cells.

Identification of LACTB2, a metallo-beta-lactamase protein, as a human mitochondrial endoribonuclease.,Levy S, Allerston CK, Liveanu V, Habib MR, Gileadi O, Schuster G Nucleic Acids Res. 2016 Jan 29. pii: gkw050. PMID:26826708<ref>PMID:26826708</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ad9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Allerston CK]]
[[Category: Arrowsmith CH]]
[[Category: Bountra C]]
[[Category: Burgess Brown N]]
[[Category: Chalk R]]
[[Category: Edwards A]]
[[Category: Elkins JM]]
[[Category: Filippakopoulos P]]
[[Category: Gileadi O]]
[[Category: Krojer T]]
[[Category: Muniz JRC]]
[[Category: Pike ACW]]
[[Category: Shrestha B]]
[[Category: Vollmar M]]
[[Category: Weigelt J]]
[[Category: Von Delft F]]