View source for 469d


==CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA==
<StructureSection load='469d' size='340' side='right' caption='[[469d]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[469d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=469D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=469D FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A44:2-O-METHYOXYETHYL-ADENOSINE+5-MONOPHOSPHATE'>A44</scene>, <scene name='pdbligand=C43:2-O-METHYOXYETHYL-CYTIDINE-5-MONOPHOSPHATE'>C43</scene>, <scene name='pdbligand=G48:2-O-METHYOXYETHYL-GUANOSINE-5-MONOPHOSPHATE'>G48</scene>, <scene name='pdbligand=U36:2-O-METHYOXYETHYL-URIDINE-5-MONOPHOSPHATE'>U36</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=469d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=469d OCA], [http://pdbe.org/469d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=469d RCSB], [http://www.ebi.ac.uk/pdbsum/469d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=469d ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance.

Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA.,Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:10360355<ref>PMID:10360355</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 469d" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cook, P D]]
[[Category: Egli, M]]
[[Category: Inamati, G]]
[[Category: Minasov, G]]
[[Category: Teplova, M]]
[[Category: Tereshko, V]]
[[Category: Rna]]
[[Category: Rna dodecamer duplex]]