View source for 3waf


==X-ray structure of apo-TtFbpA, a ferric ion-binding protein from thermus thermophilus HB8==
<StructureSection load='3waf' size='340' side='right'caption='[[3waf]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3waf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WAF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WAF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3waf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3waf OCA], [https://pdbe.org/3waf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3waf RCSB], [https://www.ebi.ac.uk/pdbsum/3waf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3waf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5SHV2_THET8 Q5SHV2_THET8] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Crystal structures of FbpA, the periplasmic ferric ion-binding protein of an iron-uptake ABC transporter, from Thermus thermophilus HB8 (TtFbpA) have been solved in apo and ferric ion-bound forms at 1.8 and 1.7 A resolution, respectively. The latter crystal structure shows that the bound ferric ion forms a novel six-coordinated complex with three tyrosine side chains, two bicarbonates and a water molecule in the metal-binding site. The results of gel-filtration chromatography and dynamic light scattering show that TtFbpA exists as a monomer in solution regardless of ferric ion binding and that TtFbpA adopts a more compact conformation in the ferric ion-bound state than in the apo state in solution.

A novel mode of ferric ion coordination by the periplasmic ferric ion-binding subunit FbpA of an ABC-type iron transporter from Thermus thermophilus HB8.,Wang S, Ogata M, Horita S, Ohtsuka J, Nagata K, Tanokura M Acta Crystallogr D Biol Crystallogr. 2014 Jan;70(Pt 1):196-202. doi:, 10.1107/S1399004713026333. Epub 2013 Dec 31. PMID:24419392<ref>PMID:24419392</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3waf" style="background-color:#fffaf0;"></div>

==See Also==
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB8]]
[[Category: Horita S]]
[[Category: Nagata K]]
[[Category: Ogata M]]
[[Category: Ohtsuka J]]
[[Category: Tanokura M]]
[[Category: Wang S]]