View source for 3mak


==Crystal structure of Glutathione transferase dmGSTD1 from Drosophila melanogaster, in complex with glutathione==
<StructureSection load='3mak' size='340' side='right'caption='[[3mak]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3mak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MAK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mak OCA], [https://pdbe.org/3mak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mak RCSB], [https://www.ebi.ac.uk/pdbsum/3mak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mak ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GSTD1_DROME GSTD1_DROME] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028, PubMed:20417639). Has DDT dehydrochlorinase activity (PubMed:20417639). May be involved in detoxification (PubMed:22082028).<ref>PMID:20417639</ref> <ref>PMID:22082028</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report four new crystal structures for Delta class glutathione transferases from insects. We compare these new structures as well as several previously reported structures to determine that structural transitions can be observed with ligand binding. These transitions occurred in the regions around the active site entrance, including alpha helix 2, C-terminus of alpha helix 4 including the loop to helix 5 and the C-terminus of helix 8. These structural movements have been reported or postulated to occur for several other glutathione transferase classes; however, this is the first report showing structural evidence of all these movements occurring, in this case in Delta class glutathione transferases. These fluctuations also can be observed occurring within a single structure as there is ligand bound in only one subunit and each subunit is undergoing different conformational transitions. The structural comparisons show reorganizations occur both pre- and post-GSH ligand binding communicated through the subunit interface of the quaternary assembly. Movements of these positions would allow 'breathing' of the active site for substrate entrance, topological rearrangement for varying substrate specificity and final product release.

Structural evidence for conformational changes of Delta class glutathione transferases after ligand binding.,Wongsantichon J, Robinson RC, Ketterman AJ Arch Biochem Biophys. 2012 May;521(1-2):77-83. doi: 10.1016/j.abb.2012.03.023., Epub 2012 Mar 27. PMID:22475449<ref>PMID:22475449</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3mak" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Ketterman AJ]]
[[Category: Robinson RC]]
[[Category: Wongsantichon J]]