View source for 2wwb


==CRYO-EM STRUCTURE OF THE MAMMALIAN SEC61 COMPLEX BOUND TO THE ACTIVELY TRANSLATING WHEAT GERM 80S RIBOSOME==
<SX load='2wwb' size='340' side='right' viewer='molstar' caption='[[2wwb]], [[Resolution|resolution]] 6.48&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2wwb]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WWB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.48&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wwb OCA], [https://pdbe.org/2wwb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wwb RCSB], [https://www.ebi.ac.uk/pdbsum/2wwb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wwb ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/S61A1_CANLF S61A1_CANLF] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ww/2wwb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wwb ConSurf].
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== Publication Abstract from PubMed ==
The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that a single copy may serve as an active PCC. We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. In combination with biochemical data, we found that in both idle and active states, the Sec complex is not oligomeric and interacts mainly via two cytoplasmic loops with the universal ribosomal adaptor site. In the active state, the ribosomal tunnel and a central pore of the monomeric PCC were occupied by the nascent chain, contacting loop 6 of the Sec complex. This provides a structural basis for the activity of a solitary Sec complex in cotranslational protein translocation.

Structure of monomeric yeast and Mammalian sec61 complexes interacting with the translating ribosome.,Becker T, Bhushan S, Jarasch A, Armache JP, Funes S, Jossinet F, Gumbart J, Mielke T, Berninghausen O, Schulten K, Westhof E, Gilmore R, Mandon EC, Beckmann R Science. 2009 Dec 4;326(5958):1369-73. Epub 2009 Oct 29. PMID:19933108<ref>PMID:19933108</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2wwb" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosomal protein L17|Ribosomal protein L17]]
*[[Ribosomal protein L19|Ribosomal protein L19]]
*[[Ribosomal protein L23|Ribosomal protein L23]]
*[[Ribosomal protein L25|Ribosomal protein L25]]
*[[Ribosomal protein L26|Ribosomal protein L26]]
*[[Ribosomal protein L31|Ribosomal protein L31]]
*[[Ribosomal protein L35|Ribosomal protein L35]]
*[[Ribosomal protein L39|Ribosomal protein L39]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Canis lupus familiaris]]
[[Category: Large Structures]]
[[Category: Triticum aestivum]]
[[Category: Armache JP]]
[[Category: Becker T]]
[[Category: Beckmann R]]
[[Category: Berninghausen O]]
[[Category: Bhushan S]]
[[Category: Funes S]]
[[Category: Gilmore R]]
[[Category: Gumbart J]]
[[Category: Jarasch A]]
[[Category: Jossinet F]]
[[Category: Mandon E]]
[[Category: Mielke T]]
[[Category: Schulten K]]
[[Category: Westhof E]]