View source for 2mw7


==Solution NMR structure of a novel cysteine framework containing Conus peptide Mo3964==
<StructureSection load='2mw7' size='340' side='right'caption='[[2mw7]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2mw7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_monile Conus monile]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MW7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mw7 OCA], [https://pdbe.org/2mw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mw7 RCSB], [https://www.ebi.ac.uk/pdbsum/2mw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mw7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CXRA_CONMO CXRA_CONMO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of a new cysteine framework (-C-CC-C-C-C-) "M"-superfamily conotoxin, Mo3964, shows it to have a beta-sandwich structure that is stabilized by inter-sheet cross disulfide bonds. Mo3964 decreases outward K(+) currents in rat dorsal root ganglion neurons and increases the reversal potential of the NaV1.2 channels. The structure of Mo3964 (PDB ID: 2MW7 ) is constructed from the disulfide connectivity pattern, i.e., 1-3, 2-5, and 4-6, that is hitherto undescribed for the "M"-superfamily conotoxins. The tertiary structural fold has not been described for any of the known conus peptides. NOE (549), dihedral angle (84), and hydrogen bond (28) restraints, obtained by measurement of (h3)JNC' scalar couplings, were used as input for structure calculation. The ensemble of structures showed a backbone root mean square deviation of 0.68 +/- 0.18 A, with 87% and 13% of the backbone dihedral (varphi, psi) angles lying in the most favored and additional allowed regions of the Ramachandran map. The conotoxin Mo3964 represents a new bioactive peptide fold that is stabilized by disulfide bonds and adds to the existing repertoire of scaffolds that can be used to design stable bioactive peptide molecules.

A Disulfide Stabilized beta-Sandwich Defines the Structure of a New Cysteine Framework M-Superfamily Conotoxin.,Kancherla AK, Meesala S, Jorwal P, Palanisamy R, Sikdar SK, Sarma SP ACS Chem Biol. 2015 Aug 21;10(8):1847-60. doi: 10.1021/acschembio.5b00226. Epub, 2015 May 18. PMID:25961405<ref>PMID:25961405</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mw7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Conus monile]]
[[Category: Large Structures]]
[[Category: Kancherla A]]
[[Category: Sarma SP]]