View source for 2mar


==Solution structure of Ani s 5 Anisakis simplex allergen==
<StructureSection load='2mar' size='340' side='right'caption='[[2mar]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2mar]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Anisakis_simplex Anisakis simplex]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MAR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mar OCA], [https://pdbe.org/2mar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mar RCSB], [https://www.ebi.ac.uk/pdbsum/2mar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mar ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ANIS5_ANISI ANIS5_ANISI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: Anisakiasis is a re-emerging global disease caused by consumption of raw or lightly cooked fish contaminated with L3 Anisakis larvae. This zoonotic disease is characterized by severe gastrointestinal and/or allergic symptoms which may misdiagnosed as appendicitis, gastric ulcer or other food allergies. The Anisakis allergen Ani s 5 is a protein belonging to the SXP/RAL-2 family; it is detected exclusively in nematodes. Previous studies showed that SXP/RAL-2 proteins are active antigens; however, their structure and function remain unknown. The aim of this study was to elucidate the three-dimensional structure of Ani s 5 and its main IgE and IgG4 binding regions. METHODOLOGY/PRINCIPAL FINDINGS: The tertiary structure of recombinant Ani s 5 in solution was solved by nuclear magnetic resonance. Mg2+, but not Ca2+, binding was determined by band shift using SDS-PAGE. IgE and IgG4 epitopes were elucidated by microarray immunoassay and SPOTs membranes using sera from nine Anisakis allergic patients. The tertiary structure of Ani s 5 is composed of six alpha helices (H), with a Calmodulin like fold. H3 is a long, central helix that organizes the structure, with H1 and H2 packing at its N-terminus and H4 and H5 packing at its C-terminus. The orientation of H6 is undefined. Regarding epitopes recognized by IgE and IgG4 immunoglobulins, the same eleven peptides derived from Ani s 5 were bound by both IgE and IgG4. Peptides 14 (L40-K59), 26 (A76-A95) and 35 (I103-D122) were recognized by three out of nine sera. CONCLUSIONS/SIGNIFICANCE: This is the first reported 3D structure of an Anisakis allergen. Magnesium ion binding and structural resemblance to Calmodulin, suggest some putative functions for SXP/RAL-2 proteins. Furthermore, the IgE/IgG4 binding regions of Ani s 5 were identified as segments localized on its surface. These data will contribute towards a better understanding of the interactions that occur between immunoglobulins and allergens and, in turn, facilitate the design of novel diagnostic tests and immunotherapeutic strategies.

Relationships between IgE/IgG4 epitopes, structure and function in Anisakis simplex Ani s 5, a member of the SXP/RAL-2 protein family.,Garcia-Mayoral MF, Trevino MA, Perez-Pinar T, Caballero ML, Knaute T, Umpierrez A, Bruix M, Rodriguez-Perez R PLoS Negl Trop Dis. 2014 Mar 6;8(3):e2735. doi: 10.1371/journal.pntd.0002735., eCollection 2014 Mar. PMID:24603892<ref>PMID:24603892</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mar" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Anisakis simplex]]
[[Category: Large Structures]]
[[Category: Bruix M]]
[[Category: Caballero ML]]
[[Category: Garcia-Mayoral MF]]
[[Category: Knaute T]]
[[Category: Perez-Pinar T]]
[[Category: Rodriguez-Perez R]]
[[Category: Trevino MA]]
[[Category: Umpierrez A]]