View source for 2dpf


==Crystal Structure of curculin1 homodimer==
<StructureSection load='2dpf' size='340' side='right'caption='[[2dpf]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2dpf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Molineria_latifolia Molineria latifolia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DPF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dpf OCA], [https://pdbe.org/2dpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dpf RCSB], [https://www.ebi.ac.uk/pdbsum/2dpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dpf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CURC1_MOLLA CURC1_MOLLA] Taste-modifying protein; sweet-tasting. After curculin, water elicits a sweet taste, and sour substances induce a stronger sense of sweetness.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/2dpf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dpf ConSurf].
<div style="clear:both"></div>
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== Publication Abstract from PubMed ==
Curculin isolated from Curculigo latifolia, a plant grown in Malaysia, has an intriguing property of modifying sour taste into sweet taste. In addition to this taste-modifying activity, curculin itself elicits a sweet taste. Although these activities have been attributed to the heterodimeric isoform and not homodimers of curculin, the underlying mechanisms for the dual action of this protein have been largely unknown. To identify critical sites for these activities, we performed a mutational and structural study of recombinant curculin. Based on the comparison of crystal structures of curculin homo- and heterodimers, a series of mutants was designed and subjected to tasting assays. Mapping of amino acid residues on the three-dimensional structure according to their mutational effects revealed that the curculin heterodimer exhibits sweet-tasting and taste-modifying activities through its partially overlapping but distinct molecular surfaces. These findings suggest that the two activities of the curculin heterodimer are expressed through its two different modes of interactions with the T1R2-T1R3 heterodimeric sweet taste receptor.

Curculin exhibits sweet-tasting and taste-modifying activities through its distinct molecular surfaces.,Kurimoto E, Suzuki M, Amemiya E, Yamaguchi Y, Nirasawa S, Shimba N, Xu N, Kashiwagi T, Kawai M, Suzuki E, Kato K J Biol Chem. 2007 Nov 16;282(46):33252-6. Epub 2007 Sep 25. PMID:17895249<ref>PMID:17895249</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2dpf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Molineria latifolia]]
[[Category: Amemiya E]]
[[Category: Kashiwagi T]]
[[Category: Kato K]]
[[Category: Kawai M]]
[[Category: Kurimoto E]]
[[Category: Nirasawa S]]
[[Category: Shimba N]]
[[Category: Suzuki E]]
[[Category: Suzuki M]]
[[Category: Xu N]]
[[Category: Yamaguchi Y]]