View source for 2bun


==Solution structure of the BLUF domain of AppA 5-125==
<StructureSection load='2bun' size='340' side='right'caption='[[2bun]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2bun]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides_2.4.1 Cereibacter sphaeroides 2.4.1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BUN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bun OCA], [https://pdbe.org/2bun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bun RCSB], [https://www.ebi.ac.uk/pdbsum/2bun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bun ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q53119_CERSP Q53119_CERSP] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bu/2bun_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bun ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The transcriptional antirepressor AppA from the photosynthetic bacterium Rhodobacter sphaeroides senses both the light climate and the intracellular redox state. Under aerobic conditions in the dark, AppA binds to and thereby blocks the function of PpsR, a transcriptional repressor. Absorption of a blue photon dissociates AppA from PpsR and allows the latter to repress photosynthesis gene expression. The N terminus of AppA contains sequence homology to flavin-containing photoreceptors that belong to the BLUF family. Structural and chemical aspects of signal transduction mediated by AppA are still largely unknown. Here we present NMR studies of the N-terminal flavin-binding BLUF domain of AppA. Its solution structure adopts an alpha/beta-sandwich fold with a beta alpha beta beta alpha beta beta topology, which represents a new flavin-binding fold. Considerable disorder is observed for residues near the chromophore due to conformational exchange. This disorder is observed both in the dark and in the light-induced signaling state of AppA. Furthermore, we detect light-induced structural changes in a patch of surface residues that provide a structural link between light absorption and signal-transduction events.

The solution structure of the AppA BLUF domain: insight into the mechanism of light-induced signaling.,Grinstead JS, Hsu ST, Laan W, Bonvin AM, Hellingwerf KJ, Boelens R, Kaptein R Chembiochem. 2006 Jan;7(1):187-93. PMID:16323221<ref>PMID:16323221</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2bun" style="background-color:#fffaf0;"></div>

==See Also==
*[[AppA protein BLUF domain|AppA protein BLUF domain]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cereibacter sphaeroides 2 4.1]]
[[Category: Large Structures]]
[[Category: Boelens R]]
[[Category: Bonvin AMJJ]]
[[Category: Grinstead JS]]
[[Category: Hellingwerf KJ]]
[[Category: Hsu S-T]]
[[Category: Kaptein R]]
[[Category: Laan W]]