View source for 1lmv


==Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae==
<StructureSection load='1lmv' size='340' side='right'caption='[[1lmv]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1lmv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LMV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lmv OCA], [https://pdbe.org/1lmv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lmv RCSB], [https://www.ebi.ac.uk/pdbsum/1lmv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lmv ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing.

Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine.,Newby MI, Greenbaum NL Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583<ref>PMID:12426583</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Greenbaum NL]]
[[Category: Newby MI]]