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[[Image:2igh.jpg|left|200px]]<br /><applet load="2igh" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2igh" />
'''DETERMINATION OF THE SOLUTION STRUCTURES OF DOMAINS II AND III OF PROTEIN G FROM STREPTOCOCCUS BY 1H NMR'''<br />

==Overview==
We have used 1H nuclear magnetic resonance spectroscopy to determine the, solution structures of two small (61 and 64 residue) immunoglobulin G, (IgG)-binding domains from protein G, a cell-surface protein from, Streptococcus strain G148. The two domains differ in sequence by four, amino acid substitutions, and differ in their affinity for some subclasses, of IgG. The structure of domain II was determined using a total of 478, distance restraints, 31 phi and 9 chi 1 dihedral angle restraints; that of, domain III was determined using a total of 445 distance restraints, 31 phi, and 9 chi 1 dihedral angle restraints. A protocol which involved distance, geometry, simulated annealing and restrained molecular dynamics was used, to determine ensembles of 40 structures consistent with these restraints., The structures are found to consist of an alpha-helix packed against a, four-stranded antiparallel-parallel-antiparallel beta-sheet. The, structures of the two domains are compared to each other and to the, reported structure of a similar domain from a protein G from a different, strain of Streptococcus. We conclude that the difference in affinity of, domains II and III for IgG is due to local changes in amino acid, side-chains, rather than a more extensive change in conformation, suggesting that one or more of the residues which differ between them are, directly involved in interaction with IgG.

==About this Structure==
2IGH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._gx7805 Streptococcus sp. gx7805]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IGH OCA]. 

==Reference==
Determination of the solution structures of domains II and III of protein G from Streptococcus by 1H nuclear magnetic resonance., Lian LY, Derrick JP, Sutcliffe MJ, Yang JC, Roberts GC, J Mol Biol. 1992 Dec 20;228(4):1219-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1474588 1474588]
[[Category: Single protein]]
[[Category: Streptococcus sp. gx7805]]
[[Category: Derrick, J.P.]]
[[Category: Lian, L.Y.]]
[[Category: Roberts, G.C.K.]]
[[Category: Sutcliffe, M.J.]]
[[Category: Yang, J.C.]]
[[Category: immunoglobulin-binding protein]]

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