View source for 2fwu

[[Image:2fwu.gif|left|200px]]<br /><applet load="2fwu" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2fwu" />
'''Second Ca2+ binding domain of the Na,Ca-exchanger (NCX1)'''<br />

==Overview==
The plasma membrane Na+/Ca2+ exchanger (NCX) is almost certainly the major, Ca2+ extrusion mechanism in cardiac myocytes. Binding of Na+ and Ca2+ ions, to its large cytosolic loop regulates ion transport of the exchanger. We, determined the solution structures of two Ca2+ binding domains (CBD1 and, CBD2) that, together with an alpha-catenin-like domain (CLD), form the, regulatory exchanger loop. CBD1 and CBD2 are very similar in the Ca2+, bound state and describe the Calx-beta motif. Strikingly, in the absence, of Ca2+, the upper half of CBD1 unfolds while CBD2 maintains its, structural integrity. Together with a 7-fold higher affinity for Ca2+, this suggests that CBD1 is the primary Ca2+ sensor. Specific point, mutations in either domain largely allow the interchange of their, functionality and uncover the mechanism underlying Ca2+ sensing in NCX.

==About this Structure==
2FWU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FWU OCA]. 

==Reference==
Ca2+ regulation in the Na+/Ca2+ exchanger involves two markedly different Ca2+ sensors., Hilge M, Aelen J, Vuister GW, Mol Cell. 2006 Apr 7;22(1):15-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16600866 16600866]
[[Category: Canis lupus familiaris]]
[[Category: Single protein]]
[[Category: Aelen, J.]]
[[Category: Hilge, M.]]
[[Category: Vuister, G.W.]]
[[Category: CA]]
[[Category: beta-bulge]]
[[Category: beta-sandwich]]
[[Category: ca2+ binding]]
[[Category: greek key]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:49:05 2007''