View source for 1gce

[[Image:1gce.gif|left|200px]]<br />
<applet load="1gce" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1gce, resolution 1.8&Aring;" />
'''STRUCTURE OF THE BETA-LACTAMASE OF ENTEROBACTER CLOACAE GC1'''<br />

==Overview==
A class C beta-lactamase from a clinical isolate of Enterobacter cloacae, strain GC1 with improved hydrolytic activity for oxyimino beta-lactam, antibiotics has been analyzed by X-ray crystallography to 1.8 A, resolution. Relative to the wild-type P99 beta-lactamase, this natural, mutant contains a highly unique tandem repeat Ala211-Val212-Arg213 [Nugaka, et al. (1995) J. Biol. Chem. 270, 5729-5735]. The 39.4 kDa chromosomal, beta-lactamase crystallizes from poly(ethylene glycol) 8000 in potassium, phosphate in space group P2(1)2(1)2 with cell dimensions a = 78.0 A, b =, 69.5 A, and c = 63.1 A. The crystal structure was solved by the molecular, replacement method, and the model has been refined to an R-factor of 0.20, for all nonzero data from 8 to 1.8 A. Deviations of model bonds and ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10441119 (full description)]]

==About this Structure==
1GCE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GCE OCA]]. 

==Reference==
Structure of the extended-spectrum class C beta-lactamase of Enterobacter cloacae GC1, a natural mutant with a tandem tripeptide insertion., Crichlow GV, Kuzin AP, Nukaga M, Mayama K, Sawai T, Knox JR, Biochemistry. 1999 Aug 10;38(32):10256-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10441119 10441119]
[[Category: Enterobacter cloacae]]
[[Category: Single protein]]
[[Category: Crichlow, G.V.]]
[[Category: Knox, J.R.]]
[[Category: Kuzin, A.P.]]
[[Category: Nukaga, M.]]
[[Category: Sawai, T.]]
[[Category: beta-lactam hydrolase]]
[[Category: cephalosporinase]]
[[Category: drug design]]
[[Category: extended-spectrum beta- lactamase]]

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