View source for 2dys

[[Image:2dys.jpg|left|200px]]<br /><applet load="2dys" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2dys, resolution 2.20&Aring;" />
'''Bovine heart cytochrome C oxidase modified by DCCD'''<br />

==Overview==
All 13 lipids, including two cardiolipins, one phosphatidylcholine, three, phosphatidylethanolamines, four phosphatidylglycerols and three, triglycerides, were identified in a crystalline bovine heart cytochrome c, oxidase (CcO) preparation. The chain lengths and unsaturated bond, positions of the fatty acid moieties determined by mass spectrometry, suggest that each lipid head group identifies its specific binding site, within CcOs. The X-ray structure demonstrates that the flexibility of the, fatty acid tails facilitates their effective space-filling functions and, that the four phospholipids stabilize the CcO dimer. Binding of, dicyclohexylcarbodiimide to the O(2) transfer pathway of CcO causes two, palmitate tails of phosphatidylglycerols to block the pathway, suggesting, that the palmitates control the O(2) transfer process.The, phosphatidylglycerol with vaccenate (cis-Delta(11)-octadecenoate) was, found in CcOs of bovine and Paracoccus denitrificans, the ancestor of, mitochondrion, indicating that the vaccenate is conserved in bovine CcO in, spite of the abundance of oleate (cis-Delta(9)-octadecenoate). The X-ray, structure indicates that the protein moiety selects cis-vaccenate near the, O(2) transfer pathway against trans-vaccenate. These results suggest that, vaccenate plays a critical role in the O(2) transfer mechanism.

==About this Structure==
2DYS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with DMU, CU, ZN, MG, NA, HEA, CUA, UNX, TGL, PGV, CHD, PSC, PEK and CDL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytochrome-c_oxidase Cytochrome-c oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.1 1.9.3.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DYS OCA]. 

==Reference==
Structures and physiological roles of 13 integral lipids of bovine heart cytochrome c oxidase., Shinzawa-Itoh K, Aoyama H, Muramoto K, Terada H, Kurauchi T, Tadehara Y, Yamasaki A, Sugimura T, Kurono S, Tsujimoto K, Mizushima T, Yamashita E, Tsukihara T, Yoshikawa S, EMBO J. 2007 Mar 21;26(6):1713-25. Epub 2007 Mar 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17332748 17332748]
[[Category: Bos taurus]]
[[Category: Cytochrome-c oxidase]]
[[Category: Protein complex]]
[[Category: Aoyama, H.]]
[[Category: Kurauchi, T.]]
[[Category: Mizushima, T.]]
[[Category: Muramoto, K.]]
[[Category: Shinzawa-Itoh, K.]]
[[Category: Tsukihara, T.]]
[[Category: Yamashita, E.]]
[[Category: Yoshikawa, S.]]
[[Category: CDL]]
[[Category: CHD]]
[[Category: CU]]
[[Category: CUA]]
[[Category: DMU]]
[[Category: HEA]]
[[Category: MG]]
[[Category: NA]]
[[Category: PEK]]
[[Category: PGV]]
[[Category: PSC]]
[[Category: TGL]]
[[Category: UNX]]
[[Category: ZN]]
[[Category: oxidoreductase]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:52:00 2007''