View source for 2bnx

[[Image:2bnx.gif|left|200px]]<br /><applet load="2bnx" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2bnx, resolution 2.40&Aring;" />
'''CRYSTAL STRUCTURE OF THE DIMERIC REGULATORY DOMAIN OF MOUSE DIAPHANEOUS-RELATED FORMIN (DRF), MDIA1'''<br />

==Overview==
Diaphanous-related formins (DRFs) regulate dynamics of unbranched actin, filaments during cell contraction and cytokinesis. DRFs are autoinhibited, through intramolecular binding of a Diaphanous autoinhibitory domain (DAD), to a conserved N-terminal regulatory element. Autoinhibition is relieved, through binding of the GTPase RhoA to the N-terminal element. We report, the crystal structure of the dimeric regulatory domain of the DRF, mDia1., Dimerization is mediated by an intertwined six-helix bundle, from which, extend two Diaphanous inhibitory domains (DIDs) composed of five armadillo, repeats. NMR and biochemical mapping indicate the RhoA and DAD binding, sites on the DID partially overlap, explaining activation of mDia1 by the, GTPase. RhoA binding also requires an additional structurally independent, segment adjacent to the DID. This regulatory construction, involving a, GTPase binding site spanning a flexibly tethered arm and the inhibitory, module, is observed in many autoinhibited effectors of Ras superfamily, GTPases, suggesting evolutionary pressure for this design.

==About this Structure==
2BNX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BNX OCA]. 

==Reference==
Structural basis of Rho GTPase-mediated activation of the formin mDia1., Otomo T, Otomo C, Tomchick DR, Machius M, Rosen MK, Mol Cell. 2005 Apr 29;18(3):273-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15866170 15866170]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Machius, M.]]
[[Category: Otomo, C.]]
[[Category: Otomo, T.]]
[[Category: Rosen, M.]]
[[Category: Tomchick, D.R.]]
[[Category: CL]]
[[Category: actin]]
[[Category: autoinhibition]]
[[Category: cytoskeleton]]
[[Category: nucleation]]
[[Category: structural protein]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:51:04 2007''