View source for 2amo

[[Image:2amo.gif|left|200px]]<br /><applet load="2amo" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2amo, resolution 2.60&Aring;" />
'''Loose Dimer of a Bacillus subtilis Nitric Oxide Synthase'''<br />

==Overview==
Cooperativity among ligand binding, subunit association, and protein, folding has implications for enzyme regulation as well as protein, aggregation events associated with disease. The binding of substrate, l-arginine or cofactor tetrahydrobiopterin converts nitric oxide synthases, (NOSs) from a "loose dimer", with an exposed active center and higher, sensitivity to proteolysis, to a "tight dimer" competent for catalysis., The crystallographic structure of the Bacillus subtilis NOS loose dimer, shows an altered association state with severely destabilized subdomains., Ligand binding or heme reduction converts loose dimers to tight dimers in, solution and crystals. Mutations at key positions in the dimer interface, that distinguish prokaryotic from eukaryotic NOSs affect the propensity to, form loose dimers. The loose dimer structure indicates that non-native, interactions can mediate subunit association in NOS.

==About this Structure==
2AMO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AMO OCA]. 

==Reference==
Structure of a loose dimer: an intermediate in nitric oxide synthase assembly., Pant K, Crane BR, J Mol Biol. 2005 Sep 30;352(4):932-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16126221 16126221]
[[Category: Bacillus subtilis]]
[[Category: Nitric-oxide synthase]]
[[Category: Single protein]]
[[Category: Crane, B.R.]]
[[Category: Pant, K.]]
[[Category: HEM]]
[[Category: loose dimer]]
[[Category: molten globule]]
[[Category: nitric oxide synthase]]
[[Category: non-native structure]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:13:29 2007''