View source for 2blu

[[Image:2blu.gif|left|200px]]<br />
<applet load="2blu" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2blu, resolution 1.40&Aring;" />
'''THAUMATIN AFTER A HIGH DOSE X-RAY "BURN"'''<br />

==Overview==
Specific radiation damage can be used to solve macromolecular structures, using the radiation-damage-induced phasing (RIP) method. The method has, been investigated for six disulfide-containing test structures (elastase, insulin, lysozyme, ribonuclease A, trypsin and thaumatin) using data sets, that were collected on a third-generation synchrotron undulator beamline, with a highly attenuated beam. Each crystal was exposed to the, unattenuated X-ray beam between the collection of a 'before' and an, 'after' data set. The X-ray 'burn'-induced intensity differences ranged, from 5 to 15%, depending on the protein investigated. X-ray-susceptible, substructures were determined using the integrated direct and Patterson, methods in SHELXD. The best substructures were found by downscaling the, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?16131756 (full description)]]

==About this Structure==
2BLU is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]] with TLA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BLU OCA]]. 

==Reference==
Improving radiation-damage substructures for RIP., Nanao MH, Sheldrick GM, Ravelli RB, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1227-37. Epub 2005, Aug 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16131756 16131756]
[[Category: Single protein]]
[[Category: Thaumatococcus daniellii]]
[[Category: Nanao, M.H.]]
[[Category: Ravelli, R.B.]]
[[Category: TLA]]
[[Category: phasing]]
[[Category: plant protein]]
[[Category: radiation damage]]
[[Category: rip]]
[[Category: synchrotron]]
[[Category: taste-modifying protein]]

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