View source for 1dao

[[Image:1dao.gif|left|200px]]<br />
<applet load="1dao" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1dao, resolution 3.2&Aring;" />
'''COVALENT ADDUCT OF D-AMINO ACID OXIDASE FROM PIG KIDNEY WITH 3-METHYL-2-OXO-VALERIC ACID'''<br />

==Overview==
D-Amino acid oxidase (DAAO) is the prototype of the flavin-containing, oxidases. It catalyzes the oxidative deamination of various D-amino acids, ranging from D-Ala to D-Trp. We have carried out the X-ray analysis of, reduced DAAO in complex with the reaction product imino tryptophan (iTrp), and of the covalent adduct generated by the photoinduced reaction of the, flavin with 3-methyl-2-oxobutyric acid (kVal). These structures were, solved by combination of 8-fold density averaging and least-squares, refinement techniques. The FAD redox state of DAAO crystals was assessed, by single-crystal polarized absorption microspectrophotometry. iTrp binds, to the reduced enzyme with the N, C alpha, C, and C beta atoms positioned, 3.8 A from the re side of the flavin. The indole side chain points ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9153426 (full description)]]

==About this Structure==
1DAO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with FAB as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.3 1.4.3.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DAO OCA]]. 

==Reference==
Active site plasticity in D-amino acid oxidase: a crystallographic analysis., Todone F, Vanoni MA, Mozzarelli A, Bolognesi M, Coda A, Curti B, Mattevi A, Biochemistry. 1997 May 13;36(19):5853-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9153426 9153426]
[[Category: Single protein]]
[[Category: Sus scrofa]]
[[Category: Mattevi, A.]]
[[Category: Todone, F.]]
[[Category: FAB]]
[[Category: fad cofactor]]
[[Category: flavoenzyme]]
[[Category: oxidoreductase]]

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