View source for 2fus

[[Image:2fus.gif|left|200px]]<br />
<applet load="2fus" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2fus, resolution 2.2&Aring;" />
'''MUTATIONS OF FUMARASE THAT DISTINGUISH BETWEEN THE ACTIVE SITE AND A NEARBY DICARBOXYLIC ACID BINDING SITE'''<br />

==Overview==
Two mutant forms of fumarase C from E. coli have been made using PCR and, recombinant DNA. The recombinant form of the protein included a histidine, arm on the C-terminal facilitating purification. Based on earlier studies, two different carboxylic acid binding sites, labeled A- and B-, were, observed in crystal structures of the wild type and inhibited forms of the, enzyme. A histidine at each of the sites was mutated to an asparagine., H188N at the A-site resulted in a large decrease in specific activity, while the H129N mutation at the B-site had essentially no effect. From the, results, we conclude that the A-site is indeed the active site, and a dual, role for H188 as a potential catalytic base is proposed. Crystal, structures of the two mutant proteins produced some unexpected ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9098893 (full description)]]

==About this Structure==
2FUS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with CIT as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FUS OCA]]. 

==Reference==
Mutations of fumarase that distinguish between the active site and a nearby dicarboxylic acid binding site., Weaver T, Lees M, Banaszak L, Protein Sci. 1997 Apr;6(4):834-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9098893 9098893]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Banaszak, L.J.]]
[[Category: Lees, M.]]
[[Category: Weaver, T.M.]]
[[Category: CIT]]
[[Category: carbon oxygen lyase]]
[[Category: fumarate hydratase]]
[[Category: hydrolyase]]
[[Category: kreb's cycle enzyme]]

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