View source for 1pg4

[[Image:1pg4.gif|left|200px]]<br /><applet load="1pg4" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1pg4, resolution 1.75&Aring;" />
'''Acetyl CoA Synthetase, Salmonella enterica'''<br />

==Overview==
Acetyl-coenzyme A synthetase catalyzes the two-step synthesis of, acetyl-CoA from acetate, ATP, and CoA and belongs to a family of, adenylate-forming enzymes that generate an acyl-AMP intermediate. This, family includes other acyl- and aryl-CoA synthetases, firefly luciferase, and the adenylation domains of the modular nonribosomal peptide, synthetases. We have determined the X-ray crystal structure of acetyl-CoA, synthetase complexed with adenosine-5'-propylphosphate and CoA. The, structure identifies the CoA binding pocket as well as a new conformation, for members of this enzyme family in which the approximately 110-residue, C-terminal domain exhibits a large rotation compared to structures of, peptide synthetase adenylation domains. This domain movement presents a, new set of residues to the active site and removes a conserved lysine, residue that was previously shown to be important for catalysis of the, adenylation half-reaction. Comparison of our structure with kinetic and, structural data of closely related enzymes suggests that the members of, the adenylate-forming family of enzymes may adopt two different, orientations to catalyze the two half-reactions. Additionally, we provide, a structural explanation for the recently shown control of enzyme activity, by acetylation of an active site lysine.

==About this Structure==
1PG4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica] with CL, MG, COA, PRX and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 1NNM. Active as [http://en.wikipedia.org/wiki/Acetate--CoA_ligase Acetate--CoA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.2.1.1 6.2.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PG4 OCA]. 

==Reference==
The 1.75 A crystal structure of acetyl-CoA synthetase bound to adenosine-5'-propylphosphate and coenzyme A., Gulick AM, Starai VJ, Horswill AR, Homick KM, Escalante-Semerena JC, Biochemistry. 2003 Mar 18;42(10):2866-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12627952 12627952]
[[Category: Acetate--CoA ligase]]
[[Category: Salmonella enterica]]
[[Category: Single protein]]
[[Category: Escalante-Semerena, J.C.]]
[[Category: Gulick, A.M.]]
[[Category: Homick, K.M.]]
[[Category: Horswill, A.R.]]
[[Category: Starai, V.J.]]
[[Category: CL]]
[[Category: COA]]
[[Category: EDO]]
[[Category: MG]]
[[Category: PRX]]
[[Category: amp-forming; adenylate-forming; thioester-forming]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:46:44 2007''