View source for 1opz

[[Image:1opz.gif|left|200px]]<br /><applet load="1opz" size="450" color="white" frame="true" align="right" spinBox="true" 
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'''A core mutation affecting the folding properties of a soluble domain of the ATPase protein CopA from Bacillus subtilis'''<br />

==Overview==
The two N-terminal domains of the P-type copper ATPase, CopAa and CopAb, from Bacillus subtilis differ in their folding capabilities in vitro., Whereas CopAb has the typical betaalphabetabetaalphabeta structure and is, a rigid protein, CopAa is found to be largely unfolded. A sequence, analysis of the two and of orthologue homologous proteins indicates that, Ser46 in CopAa may destabilise the hydrophobic core, as also confirmed, through a bioinformatic energy study. CopAb has a Val in the corresponding, position. The S46V and S46A mutants are found to be folded, although the, latter displays multiple conformations. S46VCopAa, in both apo and, copper(I) loaded forms, has very similar structural and dynamic properties, with respect to CopAb, besides a different length of strand beta2 and, beta4. It is intriguing that the oxygen of Thr16 is found close, though at, longer than bonding distance, to copper in both domains, as it also occurs, in a human orthologue domain. This study contributes to understanding the, behaviour of proteins that do not properly fold in vitro. A possible, biological significance of the peculiar folding behaviour of this domain, is discussed.

==About this Structure==
1OPZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Active as [http://en.wikipedia.org/wiki/Copper-exporting_ATPase Copper-exporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.4 3.6.3.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OPZ OCA]. 

==Reference==
A core mutation affecting the folding properties of a soluble domain of the ATPase protein CopA from Bacillus subtilis., Banci L, Bertini I, Ciofi-Baffoni S, Gonnelli L, Su XC, J Mol Biol. 2003 Aug 8;331(2):473-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12888353 12888353]
[[Category: Bacillus subtilis]]
[[Category: Copper-exporting ATPase]]
[[Category: Single protein]]
[[Category: Banci, L.]]
[[Category: Bertini, I.]]
[[Category: Ciofi-Baffoni, S.]]
[[Category: Gonneli, L.]]
[[Category: Su, X.C.]]
[[Category: abbab fold]]
[[Category: folding]]
[[Category: mutation]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:05:41 2007''