View source for 1ur9

[[Image:1ur9.gif|left|200px]]<br />
<applet load="1ur9" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1ur9, resolution 1.80&Aring;" />
'''INTERACTIONS OF A FAMILY 18 CHITINASE WITH THE DESIGNED INHIBITOR HM508, AND ITS DEGRADATION PRODUCT, CHITOBIONO-DELTA-LACTONE'''<br />

==Overview==
We describe enzymological and structural analyses of the interaction, between the family 18 chitinase ChiB from Serratia marcescens and the, designed inhibitor N,N'-diacetylchitobionoxime-N-phenylcarbamate (HM508)., HM508 acts as a competitive inhibitor of this enzyme with a K(i) in the 50, microM range. Active site mutants of ChiB show K(i) values ranging from 1, to 200 microM, providing insight into some of the interactions that, determine inhibitor affinity. Interestingly, the wild type enzyme slowly, degrades HM508, but the inhibitor is essentially stable in the presence of, the moderately active D142N mutant of ChiB. The crystal structure of the, D142N-HM508 complex revealed that the two sugar moieties bind to the -2, and -1 subsites, whereas the phenyl group interacts with aromatic ... [[http://ispc.weizmann.ac.il/pmbin/getpm?14597613 (full description)]]

==About this Structure==
1UR9 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]] with NAG, SO4, GDL and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UR9 OCA]]. 

==Reference==
Interactions of a family 18 chitinase with the designed inhibitor HM508 and its degradation product, chitobiono-delta-lactone., Vaaje-Kolstad G, Vasella A, Peter MG, Netter C, Houston DR, Westereng B, Synstad B, Eijsink VG, van Aalten DM, J Biol Chem. 2004 Jan 30;279(5):3612-9. Epub 2003 Nov 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14597613 14597613]
[[Category: Serratia marcescens]]
[[Category: Single protein]]
[[Category: Aalten, D.M.F.Van.]]
[[Category: Eijsink, V.G.H.]]
[[Category: Houston, D.R.]]
[[Category: Netter, C.]]
[[Category: Peter, M.G.]]
[[Category: Synstad, B.]]
[[Category: Vaaje-Kolstad, G.]]
[[Category: Vasella, A.]]
[[Category: Westereng, B.]]
[[Category: GDL]]
[[Category: GOL]]
[[Category: NAG]]
[[Category: SO4]]
[[Category: chitin degradation]]
[[Category: chitinase]]
[[Category: glycosidase]]
[[Category: hydrolase]]
[[Category: inhibition]]
[[Category: lactone]]

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