View source for 1jvo

[[Image:1jvo.gif|left|200px]]<br /><applet load="1jvo" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1jvo, resolution 2.75&Aring;" />
'''Azurin dimer, crosslinked via disulfide bridge'''<br />

==Overview==
The transfer of electrons between proteins is an essential step in, biological energy production. Two protein redox partners are often, artificially crosslinked to investigate the poorly understood mechanism by, which they interact. To better understand the effect of crosslinking on, electron transfer rates, we have constructed dimers of azurin by, crosslinking the monomers. The measured electron exchange rates, combined, with crystal structures of the dimers, demonstrate that the length of the, linker can have a dramatic effect on the structure of the dimer and the, electron transfer rate. The presence of ordered water molecules in the, protein-protein interface may considerably influence the electronic, coupling between redox centers.

==About this Structure==
1JVO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JVO OCA]. 

==Reference==
Dramatic modulation of electron transfer in protein complexes by crosslinking., van Amsterdam IM, Ubbink M, Einsle O, Messerschmidt A, Merli A, Cavazzini D, Rossi GL, Canters GW, Nat Struct Biol. 2002 Jan;9(1):48-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11740504 11740504]
[[Category: Pseudomonas aeruginosa]]
[[Category: Single protein]]
[[Category: Amsterdam, I.M.C.van.]]
[[Category: Canters, G.W.]]
[[Category: Cavazzini, D.]]
[[Category: Einsle, O.]]
[[Category: Merli, A.]]
[[Category: Messerschmidt, A.]]
[[Category: Rossi, G.L.]]
[[Category: Ubbink, M.]]
[[Category: CU]]
[[Category: covalent crosslink]]
[[Category: cupredoxin]]
[[Category: electron transfer]]

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