View source for 1i0x

[[Image:1i0x.jpg|left|200px]]<br /><applet load="1i0x" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1i0x, resolution 1.65&Aring;" />
'''RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL)'''<br />

==Overview==
In the crystalline state, ribonuclease T1 binds calcium ions at different, lattice-dependent positions. In solution, its conformational stability is, also remarkably increased in the presence of divalent metal ions., Combining urea unfolding studies and X-ray crystallography, we compared, the presence of several metal ions at specific sites in the protein to, their contribution to the overall stabilizing effect in solution. We, constructed thermodynamic cycles involving particular metal ions and, specific carboxylate functions. The resulting coupling energies indicate, that some (but not all) metal ions found at lattice contacts in crystal, structures may indeed significantly contribute to stability enhancement in, the presence of metal ions in solution.

==About this Structure==
1I0X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae] with CA and 2GP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I0X OCA]. 

==Reference==
The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution., Deswarte J, De Vos S, Langhorst U, Steyaert J, Loris R, Eur J Biochem. 2001 Jul;268(14):3993-4000. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11453993 11453993]
[[Category: Aspergillus oryzae]]
[[Category: Ribonuclease T(1)]]
[[Category: Single protein]]
[[Category: Langhorst, U.]]
[[Category: Loris, R.]]
[[Category: Steyaert, J.]]
[[Category: Swarte, J.De.]]
[[Category: Vos, S.De.]]
[[Category: 2GP]]
[[Category: CA]]
[[Category: 2'gmp]]
[[Category: metal binding]]
[[Category: ribonuclease]]
[[Category: rnase]]
[[Category: stability]]

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